NTB-A (NK-T-B Antigen/SLAM Family Member 6, SLAMF6, Activating NK receptor) (PE) | US Biological N6000-35 product information

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product summary

company name :

US Biological

product type :

antibody

product name :

NTB-A (NK-T-B Antigen/SLAM Family Member 6, SLAMF6, Activating NK receptor) (PE)

catalog :

N6000-35

quantity :

100Tests

clonality :

monoclonal

host :

mouse

conjugate :

clone name :

7H16

reactivity :

human

product information

Catalog Number :

N6000-35

Product wo Prefix :

NTB-A (NK-T-B Antigen/SLAM Family Member 6, SLAMF6, Activating NK receptor) (PE)

Host :

mouse

Product Type :

Mab

Conjugate :

Category :

Antibodies

Size1 :

100Tests

Clone # USB :

7H16

Isotype :

IgG2a

Desc1 :

NTB-A is a type I transmembrane protein belonging to the SLAM family of immune cell receptors and has been designated SLAMF6. The SLAM family proteins are members of the CD2 subgroup of the Ig superfamily. NTB-A is expressed on NK-, T-, and B cells. NTB-A exhibits homotypic interactions and can associate with adaptor molecules such as SAP to modulate immune cell functions. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul labels 1x10e6 cells Human peripheral blood lymphocytes Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Cells should be Fc-blocked by treatment with 1ug of human IgG/10e5 cells for 15 minutes at RT prior to staining. Do not wash excess blocking IgG from this reaction. 2. Transfer 25ul of the Fc-blocked cells (1x10e5 cells) or 50ul of packed whole blood to a 5ml tube. 3. Add 10ul of N6000-35. 4. Incubate for 30-45 minutes at 4°C. 5. Following this incubation, remove unreacted NTB- A/SLAMF6 reagent by washing the cells twice in 4ml of the same PBS buffer (Note: whole blood will require an RBC lysis step at this point using any commercially available lysing reagent). 6. Finally, resuspend the cells in 200-400ul of PBS buffer for final flow cytometric analysis. 7. As a control for analysis, cells in a separate tube should be treated with PE-labeled mouse IgG2a antibody. Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Stable for at 12 months at 4°C. Freezing allophycocyanin conjugates will result in a substantial loss of activity. Allophycocyanin conjugates are sensitive to light.

Calc Crossreactivity :

Immunogen :

Recombinant corresponding to Leu28-Lys225 of human NTB expressed in NSO cells. UniProt Accession: Q96DU3.

Specificity :

Recognizes human NTB-A.

Purity :

Purified by Protein A affinity chromatography

Form :

Supplied as a liquid in saline, BSA, sodium azide. Labeled with R-Phycoerythrin (PE).

Concentration :

~0.05mg/ml

Desc2 :

Product Type: Mab Isotype: IgG2a Clone No: 7H16 Host: mouse Source: human Concentration: ~0.05mg/ml Form: Supplied as a liquid in saline, BSA, sodium azide. Labeled with R-Phycoerythrin (PE). Purity: Purified by Protein A affinity chromatography Immunogen: Recombinant corresponding to Leu28-Lys225 of human NTB expressed in NSO cells. UniProt Accession: Q96DU3. Specificity: Recognizes human NTB-A. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

Storage Temperature :

4°C Do not freeze

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/13/N6000-35_1.jpg