Notch 4 (Notch Homolog 4, Drosophila, INT3) | US Biological N5375-51A product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Notch 4 (Notch Homolog 4, Drosophila, INT3)

catalog :

N5375-51A

quantity :

100 ul

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

7H44(L5C5)

reactivity :

human

product information

Catalog Number :

N5375-51A

Product wo Prefix :

Notch 4 (Notch Homolog 4, Drosophila, INT3)

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

7H44(L5C5)

Isotype :

IgG1

Desc1 :

Notch1 is a transmembrane protein functioning in development and the determination of cell-fate. During maturation, the notch molecule is cleaved by a furin-like convertase at its extracellular domain. Upon binding to a ligand such as D1, or upon extracellular calcium depletion, the carboxy-terminal notch1 fragment is released and further cleaved between Gly1743 and Val1744. The resulting activated cytosolic fragment translocates to the nucleus where it activates transcription. Notch4 is primarily expressed in endothelial cells and is processed similarly to Notch1. The mouse Notch4 gene (also known as Int-3) is a frequent target in Mouse Mammary Tumour Virus (MMTV)-induced mammary tumors. The intracellular domain of Notch4 binds to Smad3 inhibits the TGF- beta signaling. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Immunogen :

Recombinant protein consisting of the Ankyrin repeat domain of human Notch4. MW: 230kD (full length), 80kD (cleaved).

Specificity :

Recognizes levels of the transfected intracellular region of human Notch4 protein.

Purity :

Ascites

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Concentration :

Not Determined

Desc2 :

Product Type: Mab Isotype: IgG1 Clone No: 7H44(L5C5) Host: mouse Source: human Concentration: Not Determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Ascites Immunogen: Recombinant protein consisting of the Ankyrin repeat domain of human Notch4. MW: 230kD (full length), 80kD (cleaved). Specificity: Recognizes levels of the transfected intracellular region of human Notch4 protein. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

IP WB

Storage Temperature :

-20°C

Reference :

1. Artavanis-Tsakonas, S. et al. (1999) Science 284:770–776. 2. Chan, Y.M. and Jan, Y.N. (1998) Cell 94:423–426. 3. Schroeter, E.H. et al. (1998) Nature 393:382–386. 4. Rand, M.D. et al. (2000) Mol. Cell. Biol. 20:1825–1835. 5. Uyttendaele, H. et al. (1996) Development 122:2251–2259. 6. Shirayoshi, Y. et al. (1997) Genes Cells 2:213–224. 7. Baron, M. (2003) Semin. Cell Dev. Biol. 14:113–119. 8. Shirayoshi, Y. et al. (1997) Genes Cells 2:213–224. 9. Gallahan, D. and Callahan, R. (1997) Oncogene 14:1883–1890. 10. Sun, Y. et al. (2005) Oncogene 24:5365–5374.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/40/N5375-51A_1.jpg