eNOS, phosphorylated (Thr495) (Endothelial Nitric Oxide Synthase, NOS 3, NOS III) | US Biological N5350-31 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

eNOS, phosphorylated (Thr495) (Endothelial Nitric Oxide Synthase, NOS 3, NOS III)

catalog :

N5350-31

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human

product information

Catalog Number :

N5350-31

Product wo Prefix :

eNOS, phosphorylated (Thr495) (Endothelial Nitric Oxide Synthase, NOS 3, NOS III)

Host :

rabbit

Product Type :

Pab

Antigen Modification :

Phosphorylated

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

Endothelial nitric-oxide synthase (eNOS) is an important enzyme in the cardiovascular system. It catalyzes the production of nitric oxide (NO), a key regulator of blood pressure, vascular remodeling and angiogenesis (1,2). eNOS is regulated by phosphorylation at multiple sites. The two most throughly studied sites are the activation site serine 1177 and the inhibitory site threonine 495 (3). Several protein kinases including Akt/PKB, PKA and AMPK activate eNOS by phosphorylating Ser1177 in response to various stimuli (4,5). In contrast, bradykinin and hydrogen peroxide activate eNOS activity by promoting Thr495 dephosphorylation (6,7). Applications: Suitable for use in Immunoprecipitation and Western Blot. Other applications not tested. Recommended Dilution: Immunoprecipitation: 1:100 Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Bo Hu Po

Immunogen :

Synthetic phosphopeptide corresponding to residues surrounding Thr495 of human eNOS.

Specificity :

Recognizes endogenous levels of eNOS only when phosphorylated at threonine 495. Species Crossreactivity: Bovine and porcine. Reactivity is expected with human, mouse and rat due to 100% sequence homology with peptide used for immunogen.

Purity :

Purified by Protein A and peptide affinity chromatography.

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Thr495 of human eNOS. Specificity: Recognizes endogenous levels of eNOS only when phosphorylated at threonine 495. Species Crossreactivity: Bovine and porcine. Reactivity is expected with human, mouse and rat due to 100% sequence homology with peptide used for immunogen. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

IP WB

Storage Temperature :

-20°C

Reference :

1. Fulton, D., et al., J. Pharmacol. Exp. Ther. 299: 818–824 (2001). 2. Shaul, P.W., Annu. Rev. Physiol. 64: 749–774 (2002). 3. Chen, Z.P., et al., FEBS Lett. 443: 285–289 (1999). 4. Dimmeler, S., et al., Nature 399: 601–605 (1999). 5. Fulton, D., et al., Nature 399: 597–601 (1999). 6. Harris, M.B., et al., J. Biol. Chem. 276: 16,587–16,591 (2001). 7. Thomas, S.R., et al., J. Biol. Chem. 277: 6017–6024 (2002).

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/8/N5350-31_1.jpg