antibody

NOS III (Nitric Oxide Synthase 3, Nitric Oxide Synthase 3 Endothelial Cell, NOS Type III, NOS3, Endothelial Nitric Oxide Synthase 3, Endothelial NOS, eNOS, ECNOS, EC-NOS, Constitutive NOS, cNOS, Nitric Oxide Synthase Endothelial)

N5350-20F

100 ul

monoclonal

domestic rabbit

nonconjugated

7H43(49G3)

NOS III (Nitric Oxide Synthase 3, Nitric Oxide Synthase 3 Endothelial Cell, NOS Type III, NOS3, Endothelial Nitric Oxide Synthase 3, Endothelial NOS, eNOS, ECNOS, EC-NOS, Constitutive NOS, cNOS, Nitric Oxide Synthase Endothelial)

www.usbio.net/antibodies/N5350-20F/NOS-III-Nitric-Oxide-Synthase-3-Nitric-Oxide-Synthase-3-Endothelial-Cell-NOS-Type-III-NOS3-Endothelial-Nitric-Oxide-Synthase-3-Endothelial-NOS-eNOS-ECNOS-ECNOS-Constitutive-NOS-cNOS-Nitric-Oxide-Synthase-Endothelial

rabbit

Mab

Antibodies

100 ul

7H43(49G3)

IgG

Endothelial nitric-oxide synthase (NOS III, eNOS) is an important enzyme in the cardiovascular system. It catalyzes the production of nitric oxide (NO), a key regulator of blood pressure, vascular remodeling and angiogenesis (1,2). The activity of eNOS is regulated by phosphorylation at multiple sites. The two most thoroughly studied sites are the activation site Ser1177 and the inhibitory site Thr495 (3). Several protein kinases including Akt/PKB, PKA and AMPK activate eNOS by phosphorylating Ser1177 in response to various stimuli (4,5). In contrast, bradykinin and hydrogen peroxide activate eNOS activity by promoting Thr495 dephosphorylation (6,7). Applications: . Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution:. Western Blot: 1:1000. Immunoprecipitation: 1:100. Optimal dilutions to be determined by the researcher. Storage and Stability:. May be stored at 4 C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Bo Hu

Synthetic peptide corresponding to human NOS III. Species Sequence Homology: rat, mouse and canine

Recognizes endogenous levels of total human NOS III at ~140kD. Species Crossreactivity: bovine.

Purified

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Not determined

Product Type: Mab. Isotype: IgG. Clone No: 7H43(49G3). Host: rabbit. Source: human. Concentration: Not determined. Form: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Purified. Immunogen: Synthetic peptide corresponding to human NOS III. Species Sequence Homology: rat, mouse and canine. Specificity: Recognizes endogenous levels of total human NOS III at ~140kD. Species Crossreactivity: bovine. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

IP WB

-20 C

1. Fulton, D. et al. (2001) J. Pharmacol. Exp. Ther. 299, 818-824. 2. Shaul, P.W. (2002) Annu. Rev. Physiol. 64, 749-774. 3. Chen, Z.P. et al. (1999) FEBS Lett. 443, 285-289. 4. Dimmeler, S. et al. (1999) Nature 399, 601-605. 5. Fulton, D. et al. (1999) Nature 399, 597-601. 6. Harris, M.B. et al. (2001) J. Biol. Chem. 276, 16587-16591. 7. Thomas, S.R. et al. (2002) J. Biol. Chem. 277, 6017-6024.

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