antibody

NMDA 1 Receptor, phosphorylated (Ser896) (NMDA R1, N-methyl-D-aspartate)

N2904-26A

100 ul

polyclonal

domestic rabbit

nonconjugated

phosphorylated

NMDA 1 Receptor, phosphorylated (Ser896) (NMDA R1, N-methyl-D-aspartate)

rabbit

Pab

Phosphorylated

Antibodies

100 ul

IgG

N-methyl-D-aspartate receptor (NMDAR) forms a heterodimer of at least one NR1 and one NR2A-D subunit. Multiple receptor isoforms with distinct brain distributions and functional properties arise by selective splicing of the NR1 transcripts and differential expression of the NR2 subunits. NR1 subunits bind the co-agonist glycine and NR2 subunits bind the neurotransmitter glutamate. Activation of the NMDA receptor or opening of the ion channel allows flow of Na+ and Ca2+ ions into the cell, and K+ out of the cell (1). Each subunit has a cytoplasmic domain that can be directly modified by the protein kinase/phosphatase (2). PKC can phosphorylate the NR1 subunit (NMDAR1) of the receptor at Ser890/Ser896, and PKA can phosphorylate NR1 at Ser897 (3). The phosphorylation of NR1 by PKC decreases its affinity for calmodulin, thus preventing the inhibitory effect of calmodulin on NMDAR (4). The phosphorylation of NR1 by PKA probably counteracts the inhibitory effect of calcineurin on the receptor (5). NMDAR mediates long-term potentiation and slow postsynaptic excitation, which play central roles in learning, neurodevelopment, and neuroplasticity (6). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted primary antibody in TBS, 5% BSA, 0.1% Tween 20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Mo Rt

Synthetic phosphopeptide corresponding to residues surrounding Ser896 of human NMDA Receptor 1 (GluN1).

Recognizes overexpressed NMDAR1 only when phosphorylated at serine 896. Species Crossreactivity: Human, mouse and rat.

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Ser896 of human NMDA Receptor 1 (GluN1). Specificity: Recognizes overexpressed NMDAR1 only when phosphorylated at serine 896. Species Crossreactivity: Human, mouse and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

WB

-20°C

1. Liu, X.B. et al. (2004) J Neurosci 24, 8885-95. 2. Westphal, R.S. et al. (1999) Science 285, 93-6. 3. Tingley, W.G. et al. (1997) J Biol Chem 272, 5157-66. 4. Hisatsune, C. et al. (1997) J Biol Chem 272, 20805-10. 5. Raman, I.M. et al. (1996) Neuron 16, 415-21. 6. Makhinson, M. et al. (1999) J Neurosci 19, 2500-10.

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