Nicastrin | US Biological N2525-06 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Nicastrin

catalog :

N2525-06

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

N2525-06

Product wo Prefix :

Nicastrin

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

Nicastrin is a transmembrane glycoprotein serving as an essential component of the gammasecretase complex (1,2). Nicastrin protein is physically associated with presenilin and plays an important role in stabilization and correct localization of presenilin to the membrane-bound gamma-secretase complex (3). Nicastrin also serves as a docking site for gamma-secretase substrates such as APP and Notch, by directly binding to them and presenting them properly to gammasecretase to ensure the correct cleavage process (2,4). Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Hu Mk Mo Rt

Immunogen :

Synthetic peptide corresponding to residues surrounding the C-terminus of human Nicastrin (KLH).

Specificity :

Recognizes endogenous levels of human Nicastrin at 110 and 120kD. Species Crossreactivity: mouse, monkey and rat.

Purity :

Purified by Protein A and peptide affinity chromatography.

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to residues surrounding the C-terminus of human Nicastrin (KLH). Specificity: Recognizes endogenous levels of human Nicastrin at 110 and 120kD. Species Crossreactivity: mouse, monkey and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E WB

Storage Temperature :

-20°C

Reference :

1. Yu, G. et al. (2000) Nature 407, 48–54. 2. Esler, W.P. et al. (2002) Proc. Natl. Acad. Sci. USA 99, 2720–2725. 3. Kopan, R. and Goate, A. (2002) Neuron 33, 321–324. 4. Chen, F. et al. (2001) Nat. Cell Biol. 3, 751–754.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/40/N2525-06_1.jpg