antibody

Nuclear factor of activated T cells c1 (NFATC1, NFAT2a)

N2375-21B

100 ug

polyclonal

domestic goat

nonconjugated

Nuclear factor of activated T cells c1 (NFATC1, NFAT2a)

goat

Pab

Antibodies

100 ug

IgG

NFATC1 (Nuclear factor of activated T cells c1; also NFAT2a) is a 120kD member of the NFAT family of transcription factors. NFATC1 is found in T cells and mast cells, and regulates cytokine transcription. NFATC1 is normally cytoplasmic and phosphorylated at Ser172. Upon a rise in intracellular Ca +2 , dephosphorylation occurs via calcineurin, and NFATC1 enters the nucleus. Human NFATC1 is 943aa in length. It contains a calcineurin-binding site (aa118-123), two transactivation domains (aa126-218 and 703-943), two NLSs (aa265-267 and 682-684), an NES (aa310-321) and an RHD that binds DNA (aa410-592). There are multiple isoforms. Individually or in common, there is an alternate start site at Met473, a deletion of aa826-943, a 29aa substitution for aa1-42, and a 19aa substitution for aa 698-943. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml using Jurkat human acute T cell leukemia cell line, Ramos human Burkitt’s lymphoma cell line and Raji human Burkitt’s lymphoma cell line Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu

Recombinant protein corresponding to Ala48-Ser406 from human Nuclear factor of activated T cells c1 expressed in E. coli. UniProt Accession: O95644

Recognizes human Nuclear factor of activated T cells c1 at ~110-120kD.

Purified by immunoaffinity chromatography

Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.

~0.2mg/ml (after reconstitution)

Product Type: Pab Isotype: IgG Host: goat Source: human Concentration: ~0.2mg/ml (after reconstitution) Form: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS. Purity: Purified by immunoaffinity chromatography. Immunogen: Recombinant protein corresponding to Ala48-Ser406 from human Nuclear factor of activated T cells c1 expressed in E. coli. UniProt Accession: O95644 Specificity: Recognizes human Nuclear factor of activated T cells c1 at ~110-120kD. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

WB

-20°C

https://usbio-images.r.worldssl.net/prodimages/22/N2375-21B_1.jpg