antibody

NFkB, c-Rel (NF-kB, Nuclear Factor kappa B) discontinued

N2303-10D

100 ul

polyclonal

domestic rabbit

nonconjugated

NFkB, c-Rel (NF-kB, Nuclear Factor kappa B) discontinued

rabbit

Pab

Antibodies

100 ul

IgG

Transcription factors of the nuclear factor κ B (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50) and NF-κB2 (p100/p52). Both p105 and p100 are proteolyti- cally processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through an ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKalpha (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which is then translocated to the nucleus (9-11). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu Mo Rt

Synthetic peptide corresponding to residues surrounding Gly57 of human c-Rel protein (KLH- coupled).

Recongnizes endogenous levels of human total c-Rel protein. Species crossreactivity: mouse, rat. Sequence Homology: monkey, canine.

Purified by Protein A affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES pH 7.5, 0.1%, 150mM NaCl, 100ug/ml BSA and 50% glycerol.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES pH 7.5, 0.1%, 150mM NaCl, 100ug/ml BSA and 50% glycerol. Purity: Purified by Protein A affinity chromatography. Immunogen: Synthetic peptide corresponding to residues surrounding Gly57 of human c-Rel protein (KLH- coupled). Specificity: Recongnizes endogenous levels of human total c-Rel protein. Species crossreactivity: mouse, rat. Sequence Homology: monkey, canine. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

WB

-20°C

1. Baeuerle, P.A. and Henkel, T. (1994) Annu Rev Immunol 12, 141-79, 2. Baeuerle, P.A. and Baltimore, D. (1996) Cell 87, 13-20, 3. Haskill, S. et al. (1991) Cell 65, 1281-9, 4. Thompson, J.E. et al. (1995) Cell 80, 573-82, 5. Whiteside, S.T. et al. (1997) EMBO J 16, 1413-26, 6. Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83, 7. Scherer, D.C. et al. (1995) Proc Natl Acad Sci USA 92, 11259-63, 8. Chen, Z.J. et al. (1996) Cell 84, 853-62, 9. Senftleben, U. et al. (2001) Science 293, 1495-9, 10. Coope, H.J. et al. (2002) EMBO J 21, 5375-85, 11. Xiao, G. et al. (2001) Mol Cell 7, 401-9.

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