antibody

NF-kB p65, phosphorylated (Ser536) (Nuclear Factor kB) (AlexaFluor®488)

N2303-05A

100 ul

monoclonal

domestic rabbit

AF488

phosphorylated

5i125(93H1)

NF-kB p65, phosphorylated (Ser536) (Nuclear Factor kB) (AlexaFluor®488)

rabbit

Mab

Alexa Fluor®488

Phosphorylated

Antibodies

100 ul

5i125(93H1)

IgG

Transcription factors of the nuclear factor kB (NF-kB)/Rel family play a pivotal role in inflammatory and immune responses. There are five family members in mammals; RelA, c-Rel, RelB, NF-kB1 p105/p50), and NF-kB2 (p100/p52). p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. The p50 and p52 products form dimeric complexes with Rel proteins, which are then able to bind DNA and regulate transcription. In unstimulated cells, NF-kB is sequestered in the cytoplasm by its inhibitory proteins, the IkBs. NF-kBactivating agents can induce the phosphorylation of IkBs, which targets them for rapid degradation through a ubiquitin-proteasome pathway, releasing NF-kB to enter the nucleus and regulate gene expression. Processing of NF-kB2 is regulated by IKK1 (IKK-a), which triggers the phosphorylation and processing to p52, which can then undergo nuclear translocation. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:10. Add 10ul of the conjugated antibody to 500K cells in 90ul PBS/0.5% BSA. Optimal dilutions to be determined by the researcher. Storage and Stability: Store at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing Alexa Fluor®488 conjugates will result in a substantial loss of enzymatic activity.

Hm Hu Mk Mo Po Rt

Synthetic phosphopeptide corresponding to residues surrounding Ser536 of human NF-kB p65. Species sequence homology: canine

Detects human NF-kB p65 only when phosphorylated at serine 536. Does not cross-react with the p50 subunit or other related proteins. Species Crossreactivity: human, mouse, rat, hamster, monkey and porcine

Purified by immunoaffinity chromatography

Supplied as a liquid in PBS, pH 7.2, 2mg/ml BSA, 0.09% sodium azide. Labeled with AelxaFluor 488.

Not determined

Product Type: Mab Isotype: IgG Clone No: 5i125(93H1) Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in PBS, pH 7.2, 2mg/ml BSA, 0.09% sodium azide. Labeled with AelxaFluor 488. Purity: Purified by immunoaffinity chromatography. Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Ser536 of human NF-kB p65. Species sequence homology: canine Specificity: Detects human NF-kB p65 only when phosphorylated at serine 536. Does not cross-react with the p50 subunit or other related proteins. Species Crossreactivity: human, mouse, rat, hamster, monkey and porcine Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

FC

4°C Do not freeze

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