antibody

NFkB, p65, phosphorylated (Ser536) (NF-kB, Nuclear Factor kappa B, RelA)

N2302-04

100 ul

polyclonal

domestic rabbit

nonconjugated

phosphorylated

NFkB, p65, phosphorylated (Ser536) (NF-kB, Nuclear Factor kappa B, RelA)

rabbit

Pab

Phosphorylated

Antibodies

100 ul

IgG

The transcriptional nuclear factor kappaB (NF-kappaB) plays a pivotal role in inflammatory and immune responses (1,2). NF-kappaB is composed of a heterodimer of p65 and p50 subunits in most cell types and is sequestered in the cytoplasm by its inhibitory proteins, the IkappaBs (3-5). NF-kappaB-activating agents can induce the phosphorylation of IkappaBs; the phosphorylation targets them for rapid degradation through a ubiquitin-proteasome pathway, thereby releasing NF-kappaB to enter the nucleus for gene expression (6-8). It has been shown that the p65 NF-kappaB subunit is also phosphorylated during the phosphorylation and degradation of IkappaBs; the phosphorylation site is Ser536 in the C-terminal transactivation domain (9). Applications: Suitable for use in ELISA and Western Blot. Others not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu Mk Mo Rt

Synthetic phosphopeptide corresponding to residues surrounding Ser536 of human NF-kappaB p65 (KLH). Species sequence homology: canine and porcine

Detects human NF-kappaB p65 only when phosphorylated at Ser536 at 75kD. It does not crossreact with the p50 subunit or other related proteins. Species Crossreactivity: Mouse, monkey and rat

Purified by Protein A affinity chromatography.

Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added. Purity: Purified by Protein A affinity chromatography. Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Ser536 of human NF-kappaB p65 (KLH). Species sequence homology: canine and porcine Specificity: Detects human NF-kappaB p65 only when phosphorylated at Ser536 at 75kD. It does not crossreact with the p50 subunit or other related proteins. Species Crossreactivity: Mouse, monkey and rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E WB

-20°C

1. Baeuerle, P.A. and Henkel, T., (1994) Annu. Rev. Immunol. 12: 141-179. 2. Baeurle, P.A., et al., (1996) Cell 87: 13-20. 3. Haskill, S., et al., (1991) Cell 65: 1,281-1,289. 4. Thompson, J.E., et al., (1995) Cell 80: 573-582. 5. Whiteside, S.T., et al., (1997) EMBO J. 16: 1,413-1,426. 6. Traenckner, E.B., et al., (1995) EMBO J. 14: 2,876-2,883. 7. Scherer, D.C., et al., (1995) Proc. Natl. Acad. Sci. USA 92: 11,259-11,263. 8. Chen, Z.J., et al., (1996) Cell 84: 853-862. 9. Sakurai, H., et al., (1999) J. Biol. Chem. 274: 30,353-30,356.

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