antibody

Neuropeptide Y (NPY, Neuropeptide Tyrosine, Neuropeptide Y Precursor, PYY4, Y Neuropeptide)

N2176-60B

50 ul

polyclonal

domestic rabbit

nonconjugated

Neuropeptide Y (NPY, Neuropeptide Tyrosine, Neuropeptide Y Precursor, PYY4, Y Neuropeptide)

rabbit

Pab

Antibodies

50 ul

IgG

Neuropeptide Y (NPY), a 36 amino acid peptide amide, is a major regulatory neuropeptide widely distributed in the mammalian central (CNS) and peripheral nervous systems (PNS). NPY belongs to the pancreatic polypeptide family of peptides which are characterized by a common tertiary structure (1,2). Within this family, an intestinal peptide hormone, peptide YY (PYY), is most closely related to NPY. In the CNS, NPY is involved in regulation of blood pressure, memory processing, circadian rhythm, and stimulation of food intake (2). In the PNS, NPY has potent vasoconstrictor activity and acts as a neurotransmitter/neuromodulator of sympathetic neurons and adrenal glands (3,4). NPY is one of the most abundant peptides found in the CNS, widely distributed in the brain. High levels of NPY are present in the cerebral cortex, amygdaloid nuclei, hippocampal formation, and hypothalamus (5-7). In the PNS, NPY is found mainly in sympathetic neurons that innervate vascular smooth muscle, heart, and urogenital tract (3,8,9) The biological actions of NPY in the brain and periphery are mediated by at least two different NPY receptors, designated Y1 and Y2 receptor subtypes (10-11). Applications: Suitable for use in ELISA and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Indirect ELISA: 1:5000-1:10,000 using peptide coating at 0.5-1ug/well in 0.05M carbonate/bicarbonate buffer, pH 9.6 and pNPP as substrate Immunohistochemistry (Frozen): 1:8000-1:16,000 using 4% paraformaldehyde perfusion-fixed sections of rat brain Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Po Rt Sh

Synthetic porcine Neuropeptide Y (KLH) (GeneID 397304). The corresponding sequence is highly conserved (single amino acid substitution) in human and rat NPY and is highly conserved (84% identity) in the C-terminus region (24-36) of human peptide YY.

Recognizes porcine Neuropeptide Y. Crossreacts with human Peptide YY (PYY). Does not crossreact with Substance P, Neurokinin A, Neurokinin B, Calcitonin, Somatostatin or BSA. Species Crossreactivity: human, rat and sheep.

Serum

Supplied as a liquid, 15mM sodium azide.

Not Determined

Product Type: Pab Isotype: IgG Host: rabbit Source: porcine Concentration: Not Determined Form: Supplied as a liquid, 15mM sodium azide. Purity: Serum Immunogen: Synthetic porcine Neuropeptide Y (KLH) (GeneID 397304). The corresponding sequence is highly conserved (single amino acid substitution) in human and rat NPY and is highly conserved (84% identity) in the C-terminus region (24-36) of human peptide YY. Specificity: Recognizes porcine Neuropeptide Y. Crossreacts with human Peptide YY (PYY). Does not crossreact with Substance P, Neurokinin A, Neurokinin B, Calcitonin, Somatostatin or BSA. Species Crossreactivity: human, rat and sheep. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E IHC

-20°C

1. Tatemoto, K., et al., Nature, 296, 659-660 (1982). 2. Emson, P., and DeQuidt, M., Trends Neurosci., 7, 31-35 (1984). 3. Lundberg, J. M., et al., Acta Physiol. Scand., 116, 477-480 (1982). 4. Edvinsson, L., et al., Trends Pharmacol. Sci., 8, 231-235 (1987). 5. deQuidt, M. E., and Emson, P., Neurosci., 18, 545-618 (1986). 6. Higuchi, H., et al., J. Biol. Chem., 263, 6288-6295 (1988). 7. Chan-Palay, V., et al., J. Comp. Neurol., 238, 382- 389 (1985). 8. Schalling, M., et al., Neurosci., 41, 753-766 (1991). 9. Sundler, F., et al., Cell Tissue Res., 230, 487-493 (1983). 10. Michel, M. C., Trends Pharmacol. Sci., 12, 389-394 (1991). 11. Quirion, R., et al., Ann. NY Acad. Sci., 611, 58-72 (1990).