antibody

Nestin (ESTM46, FLJ21841, Intermediate Filament Protein, Nbla00170, Nes)

N2060-05A

100 ug

monoclonal

mouse

nonconjugated

3k1

The same clone is also sold as:

• Abcam: ab6320
• Invitrogen: MA1-26777

Nestin (ESTM46, FLJ21841, Intermediate Filament Protein, Nbla00170, Nes)

mouse

Mab

Antibodies

100 ug

3k1

IgG1

Nestin is a class VI intermediate filament protein that is expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Nestin expression is used extensively as a marker for CNS stem cells in the developing nervous system and in vitro cultured cells. Its transient expression is a critical step in the neural differentiation pathway. Nestin is also expressed in non-neural stem cell populations, such as pancreatic islet progenitors and hematopoietic progenitors. Applications: Suitable for use in Flow Cytometry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 2.5ug labels 10e6 cells for intracellular staining. Validated using A172 human glioblastoma cell line fixed with parafomaldehyde and permeabilized with saponin. Immunocytochemistry: 8-25ug/ml. Validated using immersion fixed human neural progenitor cells. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu

NS0 cells transfected with human Nestin.

Recognizes human Nestin.

Purified by Protein G affinity chromatography from hybridoma culture supernatant.

Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.

~0.5mg/ml (after reconstitution)

Product Type: Mab Isotype: IgG1 Clone No: 3k1 Host: mouse Source: human Concentration: ~0.5mg/ml (after reconstitution) Form: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS. Purity: Purified by Protein G affinity chromatography from hybridoma culture supernatant. Immunogen: NS0 cells transfected with human Nestin. Specificity: Recognizes human Nestin. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

FC IC

-20°C

1. Hockfield, S., et al., J. Neurosci. 5: 3310-3328 (1985). 2. Lendahl, U., et al., Cell 60: 585-595 (1990). 3. Frederiksen, K., et al., J. Neurosci. 8: 1144-1151 (1988). 4. Tohyama, T., et al., Lab. Invest. 66: 303-313 (1992). 5. Uchida, N., et al., PNAS USA 97: 14,720-14,725 (2000). 6. Frederiksen, K., et al., Neuron 1: 439-448 (1988). 7. Cattaneo, C., et al., Nature 347: 762-765 (1990). 8. Reynolds, B.A., et al., Science 255: 1707-1739 (1992). 9. Rietze, R.L., et al., Nature 412: 736-739 (2001). 10. Carpenter, M.K., et al., Exp. Neurol. 172: 383-397 (2001). 11. Zulewski, H., et al., Diabetes 50: 521-533 (2001). 12. Lumelsky, N., et al., Science 292: 1389-1394 (2001). 13. Lechner, A., et al., Biochem. Biophys. Res. Commun. 293: 670-674 (2002). 14. Shih, C.C., et al., Blood 98: 2412-2422 (2001).

https://usbio-images.r.worldssl.net/prodimages/43/N2060-05A_1.jpg