Nestin (NES, Nbla00170) | US Biological N2060-04J product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Nestin (NES, Nbla00170)

catalog :

N2060-04J

quantity :

100 ug

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

N2060-04J

Product wo Prefix :

Nestin (NES, Nbla00170)

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ug

Isotype :

IgG

Desc1 :

Nestin is a type IV intermediate filament protein expressed by neuroepithelial stem cells and which has been proposed to represent a marker for putative islet stem cells. Nestins are expressed by several types of cells during development, mostly in dividing cells of the Central Nervous System, Peripheral Nervous System and myogenic tissues. It also represents the progenitor population of neural stem cell origin. The role of nestin in dynamic cells, particularly structural organization of the cell, appears strictly regulated by phosphorylation, especially its integration into heterogeneous intermediate filaments together with vimentin or a-internexin. Nestin has recently received attention as a marker for detecting newly formed endothelial cells. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 3-5ug/ml Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Immunogen :

Synthetic peptide corresponding to aa1450-1500 from human Nestin.

Specificity :

Recognizes human Nestin. Species Crossreactivity: chimpanzee. Species sequence homology: bovine, dog, hamster, horse, mouse and rat.

Purity :

Purified by Protein A affinity chromatography.

Form :

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.

Concentration :

~0.5mg/ml

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: ~0.5mg/ml Form: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Purity: Purified by Protein A affinity chromatography. Immunogen: Synthetic peptide corresponding to aa1450-1500 from human Nestin. Specificity: Recognizes human Nestin. Species Crossreactivity: chimpanzee. Species sequence homology: bovine, dog, hamster, horse, mouse and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

IHC WB

Storage Temperature :

-20°C