Nectin-4 | US Biological N0571-60 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Nectin-4

catalog :

N0571-60

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

7H96

reactivity :

human

product information

Catalog Number :

N0571-60

Product wo Prefix :

Nectin-4

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ug

Clone # USB :

7H96

Isotype :

IgG2b

Desc1 :

Nectin-4 is a type I transmembrane glycoprotein belonging to the Nectin family of Ig superfamily proteins. It is both a homophilic and heterophilic (with Nectin-1) cell adhesion molecule that is expressed in the embryo and in breast carcinoma. A soluble form of Nectin-4 is generated from the membrane protein via the action of TACE/ADAM-17. The extracellular domain of human Nectin-4 shares 90% and 92% amino acid sequence homology with the corresponding regions of mouse and rat Nectin-4, respectively. Applications: Suitable for use in Direct ELISA, Western Blot and Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 2.5ug labels 10e6 MCF-7 human breast cancer cells. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Immunogen :

Recombinant protein corresponding to Gly27-Val351 of human Nectin 4 expressed in NS0 cells. Species Sequence Homology: mouse; 90%, rat; 92%

Specificity :

Recognizes human Nectin-4. Does not crossreact with recombinant human Nectin-1, -2, -3 or recombinant mouse Nectin-4.

Purity :

Purified by Protein A affinity chromatography from hybridoma culture supernatant

Form :

Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.

Concentration :

~0.5mg/ml (after reconstitution)

Desc2 :

Product Type: Mab Isotype: IgG2b Clone No: 7H96 Host: mouse Source: human Concentration: ~0.5mg/ml (after reconstitution) Form: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS. Purity: Purified by Protein A affinity chromatography from hybridoma culture supernatant Immunogen: Recombinant protein corresponding to Gly27-Val351 of human Nectin 4 expressed in NS0 cells. Species Sequence Homology: mouse; 90%, rat; 92% Specificity: Recognizes human Nectin-4. Does not crossreact with recombinant human Nectin-1, -2, -3 or recombinant mouse Nectin-4. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E FC WB

Storage Temperature :

-20°C