Nanog (Early Embryo Specific Expression NK Family Gene, Embryonic Stem Cell Specific Homeobox Protein, ENK, Homeobox Transcription Factor Nanog, Nanog Homeobox) | US Biological N0019-01L product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Nanog (Early Embryo Specific Expression NK Family Gene, Embryonic Stem Cell Specific Homeobox Protein, ENK, Homeobox Transcription Factor Nanog, Nanog Homeobox)

catalog :

N0019-01L

quantity :

100 ul

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

10E42(1E6C4)

reactivity :

human

product information

Catalog Number :

N0019-01L

Product wo Prefix :

Nanog (Early Embryo Specific Expression NK Family Gene, Embryonic Stem Cell Specific Homeobox Protein, ENK, Homeobox Transcription Factor Nanog, Nanog Homeobox)

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

10E42(1E6C4)

Isotype :

IgG1

Desc1 :

Nanog is a homeodomain containing transcription factor that is essential for the maintenance of pluripotency and self renewal in embryonic stem cells (1). Nanog expression is controlled by a network of factors including Sox2 and the key pluripotency regulator Oct-4 (1). Recent advances in somatic cell reprogramming have utilized viral expression of combinations of transcription factors including nanog, Oct-4, Sox2, KLF4, c-Myc and Lin28 (2,3). Applications: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry and Immunofluorescence. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunofluorescence (IF-IC): 1:2000 Immunohistochemistry (paraffin): 1:12,800 Flow Cytometry: 1:1600 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Immunogen :

Recombinant protein corresponding to human Nanog.

Specificity :

Recognizes endogenous levels of total human Nanog.

Purity :

Supernatant

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Mab Isotype: IgG1 Clone No: 10E42(1E6C4) Host: mouse Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Supernatant Immunogen: Recombinant protein corresponding to human Nanog. Specificity: Recognizes endogenous levels of total human Nanog. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

FC IF IHC WB

Storage Temperature :

-20°C

Reference :

1. Kim, J. et al. (2008) Cell 132, 1049-61, 2. Takahashi, K. et al. (2007) Nat Protoc 2, 3081-9, 3. Yu, J. et al. (2007) Science 318, 1917-20.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/31/N0019-01L_1.jpg