antibody

mTOR, phosphorylated (Ser2448) (mTAb1, Mammalian Target of Rapamycin, FRAP, RAFT)

M4696-10A

100 ul

polyclonal

domestic rabbit

nonconjugated

phosphorylated

mTOR, phosphorylated (Ser2448) (mTAb1, Mammalian Target of Rapamycin, FRAP, RAFT)

rabbit

Pab

Phosphorylated

Antibodies

100 ul

IgG

The mammalian target of rapamycin mTOR, also known as FRAP or RAFT(1-3), is a Ser/Thr protein kinase. mTOR acts as a sensor for ATP and amino acids, balancing the availability of nutrients and cell growth (4,5). When sufficient nutrients are available, mTOR responds to a phosphatidic acid-mediated signal (6), and transmits a positive signal to p70 S6 kinase, and participates in the inactivation of the eIF4E inhibitor, 4E-BP1. These events result in the translation of specific mRNA subpopulations. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481 (7,8). mTOR plays a key role in cellular growth and homeostasis, and its regulation is frequently altered in tumors. For these reasons, mTOR is currently under investigation as a potential target for anti-cancer therapy (9). Applications: Suitable for use in ELISA and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: Immunohistochemistry (Paraffin and Frozen): 1:100. Citrate/TBST Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu

Synthetic phosphopeptide corresponding to residues surrounding Ser2448 of human mTOR. Species sequence homology: mouse and rat.

Recognizes endogenous levels of human mTOR only when phosphorylated at Ser2448. MW: 289kD.

Purified by Protein A affinity chromatography.

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol, 0.02% sodium azide.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol, 0.02% sodium azide. Purity: Purified by Protein A affinity chromatography. Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Ser2448 of human mTOR. Species sequence homology: mouse and rat. Specificity: Recognizes endogenous levels of human mTOR only when phosphorylated at Ser2448. MW: 289kD. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E IHC

-20°C

1. Sabers, C.J., et al., (1995) J. Biol. Chem. 270: 815–822. 2. Brown, E.J., et al., (1994) Nature 369: 756–758. 3. Sabatini, D.M., et al., (1994) Cell 78: 35–43. 4. Gingras, A.C., et al., (2001) Genes Dev. 15: 807–826. 5. Dennis, P.B., et al., (2001) Science 294: 1,102–1,105. 6. Nave, B.T., et al., (1999) Biochem. J. 344: 427–431. 7. Peterson, R.T., et al., (2000) J. Biol. Chem. 275: 7,416–7,423. 8. Bolster, D.R., et al., (2002) J. Biol. Chem. 277: 23,977–23,980. 9. Kenerson, H.L., et al., (2002) Cancer Res. 62: 5,645-5,650.

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