antibody

mTOR, phosphorylated (Ser2448) (mTAb1, Mammalian Target of Rapamycin, FRAP, RAFT)

M4696-10

100 ul

polyclonal

domestic rabbit

nonconjugated

phosphorylated

mTOR, phosphorylated (Ser2448) (mTAb1, Mammalian Target of Rapamycin, FRAP, RAFT)

rabbit

Pab

Phosphorylated

Antibodies

100 ul

IgG

The mammalian target of rapamycin, mTOR, also know as FRAP or RAFT (1-3), is a serine/ threonine protein kinase. mTOR acts as a sensor for ATP, amino acids, balancing the availability of nutrients and cell growth (4,5). When sufficient nutrients are available, mediated by phosphatidic acid (6), mTOR transmits a positive signal to p70 S6 kinase and participates in the inactivation of the eIF4E inhibitor 4E-BP1. These events result in activation of the translation of specific mRNA subpopulations. mTOR is phosphorylated at serine 2,448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at serine 2,481 (6,7). Applications: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications have not been tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu Mk Mo Rt

Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Ser2,448 of human mTOR.

Detects endogenous levels of human mTOR only when phosphorylated at Ser2448. Species Crossreactivity: Mouse, monkey and rat.

Purified by Protein A affinity chromatography.

Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

~0.1mg/ml

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: ~0.1mg/ml Form: Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added. Purity: Purified by Protein A affinity chromatography. Immunogen: Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Ser2,448 of human mTOR. Specificity: Detects endogenous levels of human mTOR only when phosphorylated at Ser2448. Species Crossreactivity: Mouse, monkey and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E IP WB

-20°C

1. Sabers, C.J., et al., (1995) J. Biol. Chem. 270: 815–822. 2. Brown, E.J., et al., (1994) Nature 369: 756–758. 3. Sabatini, D.M., et al., (1994) Cell 78: 35–43. 4. Gingras, A.C., et al., (2001) Genes Dev. 15: 807–826. 5. Dennis, P.B., et al., (2001) Science 294: 1,102–1,105. 6. Nave, B.T., et al., (1999) Biochem. J. 344: 427–431. 7. Peterson, R.T., et al., (2000) J. Biol. Chem. 275: 7,416–7,423. 8. Bolster, D.R., et al., (2002) J. Biol. Chem. 277: 23,977–23,980. 9. Kenerson, H.L., et al., (2002) Cancer Res. 62: 5,645-5,650.

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