mTOR, phosphorylated (Ser2481) (mTAb1, Mammalian Target of Rapamycin, FRAP, RAFT) | US Biological M4696-09 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

mTOR, phosphorylated (Ser2481) (mTAb1, Mammalian Target of Rapamycin, FRAP, RAFT)

catalog :

M4696-09

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human

product information

Catalog Number :

M4696-09

Product wo Prefix :

mTOR, phosphorylated (Ser2481) (mTAb1, Mammalian Target of Rapamycin, FRAP, RAFT)

Host :

rabbit

Product Type :

Pab

Antigen Modification :

Phosphorylated

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

The mammalian target of Rapamycin, mTOR, also know as FRAP or RAFT (1-3), is a serine/ threonine protein kinase. mTOR acts as a sensor for ATP, amino acids, balancing the availability of nutrients and cell growth (4,5). When sufficient nutrients are available, mediated by phosphatidic acid (6), mTOR transmits a positive signal to p70 S6 kinase and participates in the inactivation of the eIF4E inhibitor 4E-BP1. These events result in activation of the translation of specific mRNA subpopulations. mTOR is phosphorylated at serine 2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at serine 2481 (6,7). Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Hu Mk Mo Rt

Immunogen :

Synthetic phosphopeptide corresponding to residues surrounding Ser2481 of human mTOR.

Specificity :

Detects endogenous levels of human mTOR only when phosphorylated at Ser2481. Species Crossreactivity: mouse, monkey and rat

Purity :

Purified by Protein A and peptide affinity chromatography.

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Ser2481 of human mTOR. Specificity: Detects endogenous levels of human mTOR only when phosphorylated at Ser2481. Species Crossreactivity: mouse, monkey and rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E WB

Storage Temperature :

-20°C

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/3/M4696-09_1.jpg