mTOR (mTAb1, Mammalian Target of Rapamycin, FRAP, RAFT) | US Biological M4696-05 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

mTOR (mTAb1, Mammalian Target of Rapamycin, FRAP, RAFT)

catalog :

M4696-05

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

M4696-05

Product wo Prefix :

mTOR (mTAb1, Mammalian Target of Rapamycin, FRAP, RAFT)

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

The mammalian target of rapamycin mTOR, also know as FRAP or RAFT, is a serine/ threonine protein kinase. mTOR acts as a sensor for ATP and amino acids, balancing the availability of nutrients and cell growth. When sufficient nutrients are available, mediated by phosphatidic acid, mTOR transmits a positive signal to p70 S6 kinase and participates in the inactivation of the eIF4E inhibitor 4E-BP1. These events result in activation of the translation of specific mRNA subpopulations. mTOR is phosphorylated at serine 2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at serine 2481. Applications: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Hu Mk Mo Rt

Immunogen :

Synthetic peptide (KLH coupled) corresponding to residues surrounding Ser2481 of human mTOR.

Specificity :

Detects endogenous levels of total human mTOR. It crossreacts weakly with some other proteins based on Western analysis. Species Crossreactivity: Mouse, monkey and rat.

Purity :

Purified by Protein A and peptide affinity chromatography.

Form :

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA 50% glycerol. No preservative added.

Concentration :

~0.1mg/ml

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: ~0.1mg/ml Form: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA 50% glycerol. No preservative added. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide (KLH coupled) corresponding to residues surrounding Ser2481 of human mTOR. Specificity: Detects endogenous levels of total human mTOR. It crossreacts weakly with some other proteins based on Western analysis. Species Crossreactivity: Mouse, monkey and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IP WB

Storage Temperature :

-20°C

Reference :

1. Sabers, C.J. et al. (1995) J. Biol. Chem. 270, 815–822. 2. Brown, E.J. et al. (1994) Nature 369, 756–758. 3. Sabatini, D.M. et al. (1994) Cell 78, 35–43. 4. Gingras, A.C. et al. (2001) Genes Dev. 15, 807–826. 5. Dennis, P.B. et al. (2001) Science 294, 1102–1105. 6. Nave , B.T. et al. (1999) Biochem J. 344, 427–431. 7. Peterson, R.T. et al. (2000) J. Biol. Chem. 275, 7416–7423.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/21/M4696-05_1.jpg