Moesin | US Biological M4306 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Moesin

catalog :

M4306

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

M4306

Product wo Prefix :

Moesin

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

The ezrin, radixin and moesin (ERM) proteins function as linkers between the plasma membrane and the actin cytoskeleton and are involved in cell adhesion, membrane ruffling and microvilli formation (1). ERM proteins undergo intra- or intermolecular interaction between their amino- and carboxy-terminal domains, existing as inactive, cytosolic monomers or dimers (2). Phosphorylation at a carboxy-terminal threonine residue Thr567 of ezrin, Thr564 of radixin, Thr558 of moesin), which disrupts their amino- and carboxy-terminal association, may play a key role in modulating the conformation and function of ERM proteins (3,4). Phosphorylation at Thr567 of ezrin is required for cytoskeletal rearrangements and oncogene-induced transformation (5). Ezrin is also phosphorylated at tyrosine residues upon growth factor stimulation. Phosphorylation of Tyr353 of ezrin transmits a survival signal during epithelial differentiation (6). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot. Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Bo Hu Mk Mo Rt

Immunogen :

Synthetic peptide (KLH-coupled) corresponding to residues surrounding His670 of human moesin.

Specificity :

Detects endogenous levels of total human moesin protein. The antibody does not cross-react with ezrin, radixin or other related proteins. Species Crossreactivity: bovine, mouse, monkey and rat

Purity :

Purified by Protein A and peptide affinity chromatography.

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide (KLH-coupled) corresponding to residues surrounding His670 of human moesin. Specificity: Detects endogenous levels of total human moesin protein. The antibody does not cross-react with ezrin, radixin or other related proteins. Species Crossreactivity: bovine, mouse, monkey and rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

Storage Temperature :

-20°C

Reference :

(1) Tsukita, S. and Yonemura, S. (1999) J. Biol. Chem. 274, 34507–34510. (2) Mangeat, P. et al. (1999) Trends Cell Biol. 9, 187–192. (3) Matsui, T. et al. (1998) J. Cell Biol. 140, 647–657. (4) Gautreau, A. et al. (2000) J. Cell Biol. 150, 193–203. (5) Tran Quang, C. et al. (2000) EMBO J. 19, 4565–4576. (6) Gautreau, A. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 7300–7305.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/46/M4306_1.jpg