antibody

MLK3 (Mixed Lineage Kinase 3, MLK-3, Mitogen-activated Protein Kinase Kinase Kinase 11, MAP3K11, MEKK11, PTK1, Src-homology 3 Domain-containing Proline-rich Kinase, SPRK)

M4125-05

100 ul

polyclonal

domestic rabbit

nonconjugated

MLK3 (Mixed Lineage Kinase 3, MLK-3, Mitogen-activated Protein Kinase Kinase Kinase 11, MAP3K11, MEKK11, PTK1, Src-homology 3 Domain-containing Proline-rich Kinase, SPRK)

rabbit

Pab

Antibodies

100 ul

IgG

Mixed lineage kinase 3 (MLK3) is a serine/threonine kinase that has an amino-terminal SH3 domain followed by the kinase domain and two leucine zippers, a cdc42/Rac1 binding (CRIB) domain and several other domains/motifs at the carboxy-terminal region. CRIB triggers the dimerization of MLK3 via its tandem leucine zippers, followed by the intramolecular phosphorylation and subsequent activation of MLK3 (1,2). MLK3 functions as a MAPKKK of the SAPK/JNK stress pathway by directly phosphorylating SEK1/MKK4 and MKK7, although it is controversial whether MLK3 is involved in p38 stress pathway activation (1,3). MLK3 also functions as an IkappaB kinase and mediates the activation of the transcriptional factor NF-kappaB stimulated by CD3/CD28, suggesting a role for MLK3 in immune and inflammatory responses (4). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hm Hu Mk Mo Rt

EC=2.7.11.25, MGC17114, SH3 Domain Containing Proline Rich Kinase

Synthetic peptide corresponding to residues near the C-terminal of human MLK3.

Recognizes endogenous levels of total human MLK3, ~92kD. Does not crossreact with MLK1, MLK2 and other mixed lineage kinases. Species Crossreactivity: mouse, rat, hamster and monkey.

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to residues near the C-terminal of human MLK3. Specificity: Recognizes endogenous levels of total human MLK3, ~92kD. Does not crossreact with MLK1, MLK2 and other mixed lineage kinases. Species Crossreactivity: mouse, rat, hamster and monkey. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

WB

-20°C

1. Teramoto, H. et al., J. Biol. Chem. 271: 27225-27228(1996). 2. Leung, I.W. and Lassam, N., J. Biol. Chem. 273: 32408-32415(1998). 3. Tibbles, L.A. et al., EMBO J. 15: 7026-7035(1996). 4. Hehner, S.P. et al., Mol. Cell. Biol. 20: 2556-2568(2000).

https://usbio-images.r.worldssl.net/prodimages/23/M4125-05_1.jpg