MLH1 (Human Mul L Homologue, Familial Nonpolyposis Colin Cancer Type 2, COCA2, Colon Cancer Type 2) | US Biological M3949-60C product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

MLH1 (Human Mul L Homologue, Familial Nonpolyposis Colin Cancer Type 2, COCA2, Colon Cancer Type 2)

catalog :

M3949-60C

quantity :

20 ul

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

10E39(D38G9)

reactivity :

human

product information

Catalog Number :

M3949-60C

Product wo Prefix :

MLH1 (Human Mul L Homologue, Familial Nonpolyposis Colin Cancer Type 2, COCA2, Colon Cancer Type 2)

Host :

rabbit

Product Type :

Mab

Category :

Antibodies

Size1 :

20 ul

Clone # USB :

10E39(D38G9)

Isotype :

IgG

Desc1 :

Mismatch repair (MMR), a conserved process that involves correcting errors made during DNA synthesis, is crucial to the maintenance of genomic integrity. MLH1 is the human homologue of the E. coli MMR gene mutL. MMR requires recognition of a base mismatch or insertion/deletion loop by a MutS homolog followed by recruitment of a MutL heterodimeric complex consisting of MLH1 and PMS1 (MutL-alpha), PMS2 (MutL-beta) or MLH3 (MutL-gamma). Other factors required for MMR in eukaryotes are EXO1, PCNA, RFC, RPA, DNA polymerases and DNA ligase (reviewed in 1). Inactivation of the MLH1 gene causes genome instability and redisposition to cancer (2-5). The MLH1 gene is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC) (6). MLH1 also plays a role in meiotic recombination (7). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Hu Mk

Immunogen :

Synthetic peptide corresponding to residues near the N-terminus of human MLH1.

Specificity :

Recognizes endogenous levels of human total MLH1 protein. Species crossreactivity: monkey.

Purity :

Purified

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Mab Isotype: IgG Clone No: 10E39(D38G9) Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Synthetic peptide corresponding to residues near the N-terminus of human MLH1. Specificity: Recognizes endogenous levels of human total MLH1 protein. Species crossreactivity: monkey. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

IP WB

Storage Temperature :

-20°C

Reference :

1. Modrich, P. (2006) J Biol Chem 281, 30305-9, 2. Seng, T.J. et al. (2008) Br J Cancer 99, 375-82, 3. Harley, I. et al. (2008) Gynecol Oncol 109, 384-7, 4. Mao, G. et al. (2008) J Biol Chem 283, 3211-6, 5. Hubner, R.A. and Houlston, R.S. (2007) J Natl Cancer Inst 99, 1490; author reply 1490-1, 6. Vasen, H.F. (2005) Fam Cancer 4, 219-25, 7. Argueso, J.L. et al. (2003) Mol Cell Biol 23, 873-86.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/28/M3949-60C_1.jpg