Methionine Aminopeptidase 1 (MetAP-1) | US Biological M3015-98 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Methionine Aminopeptidase 1 (MetAP-1)

catalog :

M3015-98

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

7H228

The same clone is also sold as:

MyBioSource: MBS600512

reactivity :

human

product information

Catalog Number :

M3015-98

Product wo Prefix :

Methionine Aminopeptidase 1 (MetAP-1)

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ug

Clone # USB :

7H228

Isotype :

IgG2b

Desc1 :

Methionine Aminopeptidase 1 (MetAP-1) encodes a cytoplasmic protease that removes the NH 2-terminal Met from nascent proteins. Applications: Suitable for use in Western Blot and Direct ELISA. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml using a colorimetric detection system. The detection limit is ~5ng/lane under non-reducing and reducing conditions. Direct ELISA: 0.5-1ug/ml. The detection limit for recombinant human METAP1 is ~3ng/well. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Immunogen :

Recombinant protein corresponding to aa52-386 of human Methionine Aminopeptidase 1 derived from Sf21 cells. Uniprot #P53582. Species sequence homology: chimpanzee 99%; rat/mouse 98%; chicken 95%; drosophila 64%

Specificity :

Recognizes human Methionine Aminopeptidase 1 (MetAP-1) in direct ELISAs and Western Blots. Does not crossreact with recombinant human METAP2.

Purity :

Purified by Protein G affinity chromatography.

Form :

Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.

Concentration :

~0.5mg/ml (after reconstitution)

Desc2 :

Product Type: Mab Isotype: IgG2b Clone No: 7H228 Host: mouse Source: human Concentration: ~0.5mg/ml (after reconstitution) Form: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS. Purity: Purified by Protein G affinity chromatography. Immunogen: Recombinant protein corresponding to aa52-386 of human Methionine Aminopeptidase 1 derived from Sf21 cells. Uniprot #P53582. Species sequence homology: chimpanzee 99%; rat/mouse 98%; chicken 95%; drosophila 64% Specificity: Recognizes human Methionine Aminopeptidase 1 (MetAP-1) in direct ELISAs and Western Blots. Does not crossreact with recombinant human METAP2. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E WB

Storage Temperature :

-20°C