antibody

Met, Phosphorylated (Tyr1234/1235)

M3007-21

100 ul

polyclonal

domestic rabbit

nonconjugated

phosphorylated

Met, Phosphorylated (Tyr1234/1235)

rabbit

Pab

Phosphorylated

Antibodies

100 ul

IgG

Met, a high affinity receptor for hepatocyte growth factor (HGF; also known as scatter factor), is a disulfide-linked heterodimer made of 45kD alpha and 145kD beta-subunits (1,2). The alpha-subunit and the amino-terminal region of beta-subunit form the extracellular domain. The remainder of the beta-chain spans the plasma membrane and contains a cytoplasmic region with tyrosine kinase activity. Interaction of Met with HGF results in autophosphorylation at multiple tyrosines, which recruit several downstream signaling components, including Gab1, c-Cbl and PI3 kinase (3) and is critical for all of its known biological functions. Phosphorylation of Tyr1234/1235 in the Met kinase domain is critical to kinase activation. Phosphorylation of Tyr1349 in the Met cytoplasmic domain provides a direct binding site for Gab1 (4). Altered Met levels and/or tyrosine kinase activities were found in different tumors, especially in renal, colon and breast cancers. Thus, Met is an attractive therapeutic target and cancer diagnostic (5). Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu Mk Mo Rt

Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Tyr1234/1235 of human Met.

Recognizes human Met only when phosphorylated at tyrosine 1234/1235 (Mr 145kD). This antibody crossreacts slightly with activated PDGF, insulin and FGF receptors, but it does not crossreact with other tyrosine-phosphorylated proteins. Species Crossreactivity: mouse, rat and monkey.

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Tyr1234/1235 of human Met. Specificity: Recognizes human Met only when phosphorylated at tyrosine 1234/1235 (Mr 145kD). This antibody crossreacts slightly with activated PDGF, insulin and FGF receptors, but it does not crossreact with other tyrosine-phosphorylated proteins. Species Crossreactivity: mouse, rat and monkey. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E IP WB

-20°C

1. Weidner, K.M., et al., (1993) J. Cell Biol. 121: 145-154. 2. Park, M., et al., (1986) Cell 45: 895-904. 3. Bardelli, A., et al., (1997) Oncogene 15: 3,103-3,111. 4. Schaeper, U., et al., (2000) J. Cell Biol. 149: 1,419-1,432. 5. Traxler, P., et al., (2001) Med. Res. Rev. 21: 499-512.

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