antibody

Met (Hepatocyte Growth Factor Receptor, HGF Receptor, Scatter Factor Receptor) (BSA & Azide Free)

M3007-20A

100 ul

monoclonal

mouse

nonconjugated

3i20 (25H2)

Met (Hepatocyte Growth Factor Receptor, HGF Receptor, Scatter Factor Receptor) (BSA & Azide Free)

mouse

Mab

Antibodies

100 ul

3i20 (25H2)

IgG1

Met is a high affinity receptor for hepatocyte growth factor (HGF; also known as scatter factor). Met is a disulfide-linked heterodimer made of 45kD alpha- and 145kD beta-subunits (1,2). The alpha-subunit and the amino-terminal region of beta-subunit form the extracellular domain. The remainder of the beta-chain spans the plasma membrane and contains a cytoplasmic region with tyrosine kinase activity. Interaction of Met with HGF results in autophosphorylation at multiple tyrosines, which recruit several downstream signaling components, including Gab1, c-Cbl and PI3 kinase (3). This is critical for all of its known biological functions. Phosphorylation of Tyr1234/1235 in the Met kinase domain is critical to kinase activation. Phosphorylation of Tyr1349 in the Met cytoplasmic domain provides a direct binding site for Gab1 (4). Altered Met levels and/or tyrosine kinase activities were found in different tumors, especially in renal, colon and breast cancers. Met is an attractive therapeutic target and cancer diagnostic (5). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Mk Mo Rt

Synthetic peptide corresponding to residues surrounding Tyr1234 of human Met.

Recognizes endogenous levels of human Met in various cell lines at 145kD for mature Met b-subunit and 170kD for pro-Met. Does not crossreact with any other related proteins. Species Crossreactivity: mouse, rat and monkey.

Ascites

Supplied as a liquid in PBS. No stabilizing proteins added. No preservatives added.

Not determined

Product Type: Mab Isotype: IgG1 Clone No: 3i20 (25H2) Host: mouse Source: human Concentration: Not determined Form: Supplied as a liquid in PBS. No stabilizing proteins added. No preservatives added. Purity: Ascites Immunogen: Synthetic peptide corresponding to residues surrounding Tyr1234 of human Met. Specificity: Recognizes endogenous levels of human Met in various cell lines at 145kD for mature Met b-subunit and 170kD for pro-Met. Does not crossreact with any other related proteins. Species Crossreactivity: mouse, rat and monkey. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

IP WB

-20°C

1. Weidner, K.M., et al., J. Cell Biol. 121: 145-154 (1993). 2. Park, M., et al., Cell 45: 895-904 (1986). 3. Bardelli, A., et al., Oncogene 15: 3103-3111 (1997). 4. Schaeper, U., et al., J. Cell Biol. 149: 1419-1432 (2000). 5. Traxler, P., et al., Med. Res. Rev. 21: 499-512 (2001).

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