Met (Hepatocyte Growth Factor Receptor, HGF Receptor, Scatter Factor Receptor) | US Biological M3007-20 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Met (Hepatocyte Growth Factor Receptor, HGF Receptor, Scatter Factor Receptor)

catalog :

M3007-20

quantity :

100 ul

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3i20(25H2)

reactivity :

human

product information

Catalog Number :

M3007-20

Product wo Prefix :

Met (Hepatocyte Growth Factor Receptor, HGF Receptor, Scatter Factor Receptor)

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

3i20(25H2)

Isotype :

IgG1

Desc1 :

Met is a high affinity receptor for hepatocyte growth factor (HGF; also known as scatter factor). Met is a disulfide-linked heterodimer made of 45kD alpha- and 145kD beta-subunits. The alpha-subunit and the amino-terminal region of beta-subunit form the extracellular domain. The remainder of the beta-chain spans the plasma membrane and contains a cytoplasmic region with tyrosine kinase activity. Interaction of Met with HGF results in autophosphorylation at multiple tyrosines, which recruit several downstream signaling components, including Gab1, c-Cbl and PI3 kinase. This is critical for all of its known biological functions. Phosphorylation of Tyr1234/1235 in the Met kinase domain is critical to kinase activation. Phosphorylation of Tyr1349 in the Met cytoplasmic domain provides a direct binding site for Gab1. Altered Met levels and/or tyrosine kinase activities were found in different tumors, especially in renal, colon and breast cancers. Met is an attractive therapeutic target and cancer diagnostic. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Hu Mk Mo Rt

Immunogen :

Synthetic peptide corresponding to residues surrounding Tyr1234 of human Met.

Specificity :

Recognizes endogenous levels of human Met at ~145kD and at 170kD for pro-Met. Species Crossreactivity: mouse, rat and monkey

Purity :

Ascites

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Concentration :

Not Determined

Desc2 :

Product Type: Mab Isotype: IgG1 Clone No: 3i20(25H2) Host: mouse Source: human Concentration: Not Determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Ascites Immunogen: Synthetic peptide corresponding to residues surrounding Tyr1234 of human Met. Specificity: Recognizes endogenous levels of human Met at ~145kD and at 170kD for pro-Met. Species Crossreactivity: mouse, rat and monkey Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

IP WB

Storage Temperature :

-20°C

Reference :

1. Weidner, K.M., et al., J. Cell Biol. 121: 145-154 (1993). 2. Park, M., et al., Cell 45: 895-904 (1986). 3. Bardelli, A., et al., Oncogene 15: 3103-3111 (1997). 4. Taher, T.E., et al., J. Immunol. 169: 3793-3800 (2002). 5. Schaeper, U., et al., J. Cell Biol. 149: 1419-1432 (2000). 6. Traxler, P., et al., Med. Res. Rev. 21: 499-512 (2001).

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/44/M3007-20_1.jpg