antibody

c-Met, phosphorylated (Tyr1003) (Hepatocyte Growth Factor Receptor, Scatter Factor Receptor, HGFR, SFR)

M3007-17A

100 ul

monoclonal

domestic rabbit

nonconjugated

phosphorylated

7H41(13D11)

c-Met, phosphorylated (Tyr1003) (Hepatocyte Growth Factor Receptor, Scatter Factor Receptor, HGFR, SFR)

rabbit

Mab

Phosphorylated

Antibodies

100 ul

7H41(13D11)

IgG

Met, a high affinity tyrosine kinase receptor for hepatocyte growth factor (HGF; also known as scatter factor), is a disulfide-linked heterodimer made of 45kD alpha- and 145kD beta-subunits (1,2). The alpha-subunit and the amino-terminal region of the beta-subunit form the extracellular domain. The remainder of the beta-chain spans the plasma membrane and contains a cytoplasmic region with tyrosine kinase activity. Interaction of Met with HGF results in autophosphorylation at multiple tyrosines, which recruit several downstream signaling components, including Gab1, c-Cbl and PI3 kinase (3). These are fundamental events important to all of Met's known biological functions. Addition of a phosphate at cytoplasmic Tyr1003 is essential for ubiquination and Met protein degradation (4). Phosphorylation of Tyr1234/1235 in the Met kinase domain is critical to kinase activation. Phosphorylation of Tyr1349 in the Met cytoplasmic domain provides a direct binding site for Gab1 (5). Altered Met levels and/or tyrosine kinase activities are found in several types of tumors, including renal, colon and breast cancers. Thus, Met is an attractive cancer therapeutic and diagnostic target (6). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu Mo Rt

Synthetic phosphopeptide corresponding to residues surrounding Tyr1003 of human Met.

Recognizes endogenous levels of human Met only when phosphorylated at Tyr1003. May crossreact with other activated protein tyrosine kinases. Species Crossreactivity: Mouse and rat

Purified

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Not determined

Product Type: Mab Isotype: IgG Clone No: 7H41(13D11) Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Tyr1003 of human Met. Specificity: Recognizes endogenous levels of human Met only when phosphorylated at Tyr1003. May crossreact with other activated protein tyrosine kinases. Species Crossreactivity: Mouse and rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E WB

-20°C

(1) Weidner, K.M. et al. (1993) J. Cell Biol. 121, 145–154. (2) Park, M. et al. (1986) Cell 45, 895–904. (3) Bardelli, A. et al. (1997) Oncogene 15, 3103–3111. (4) Schaeper, U. et al. (2000) J. Cell Biol. 149, 1419–1432. (5) Traxler, P. et al. (2001) Med. Res. Rev. 21, 499–512. (6) Berthou, S. et al. (2004) Oncogene 23, 5387–5393. (7) Wang, X. et al. (2003) Mol. Cancer Ther. 2, 1085–1092.

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