antibody

MerTK (c-MER, c-Mer Proto-oncogene Tyrosine Kinase, MER, MER Receptor Tyrosine Kinase, MERK, MERPEN, Proto-oncogene Tyrosine Protein Kinase MER, Receptor Tyrosine Kinase MerTK, STK Kinase)

M2995-01B

20 ul

monoclonal

domestic rabbit

nonconjugated

10g86(D21F11)

MerTK (c-MER, c-Mer Proto-oncogene Tyrosine Kinase, MER, MER Receptor Tyrosine Kinase, MERK, MERPEN, Proto-oncogene Tyrosine Protein Kinase MER, Receptor Tyrosine Kinase MerTK, STK Kinase)

rabbit

Mab

Antibodies

20 ul

10g86(D21F11)

IgG

Mer tyrosine kinase belongs to a receptor tyrosine kinase family with Axl and Tyro-3. This family is characterized by a common N-CAM (neural adhesion molecule)-related extracellular domain and a common ligand, GAS6 (growth arrest-specific protein 6). Mer protein has an apparent molecular weight of 170-210kD due to different glycosylation pattern generated in different cell types. Mer can be activated by dimerization and autophosphorylation through ligand binding or homophilic cell-cell interaction mediated by its NCAM-like motif. The downstream signaling components of activated Mer include PI3 kinase, PLCg and MAP kinase. Family members are prone to transcriptional regulation and carry out diverse functions including the regulation of cell adhesion, migration, phagocytosis, and survival. Mer regulates macrophage activation, promotes apoptotic cell engulfment, and supports platelet aggregation and clot stability in vivo. Overexpression of Mer may play a cooperative role in leukemogenesis and may be an effective target for biologically based leukemia/lymphoma therapy. Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunofluorescence/Immunocytochemistry. Other applications not tested. Recommended Dilutions: Immunofluorescence (IF-IC): 1:400 Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu

Synthetic peptide corresponding to residues surrounding His925 of human MerTK.

Recognizes endogenous levels of total human MerTK.

Purified

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Not Determined

Product Type: Mab Isotype: IgG Clone No: 10g86(D21F11) Host: rabbit Source: human Concentration: Not Determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Synthetic peptide corresponding to residues surrounding His925 of human MerTK. Specificity: Recognizes endogenous levels of total human MerTK. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

IC IF IP WB

-20°C

1. Ling, L. et al. (1996) J Biol Chem 271, 18355-62. 2. Ling, L. and Kung, H.J. (1995) Mol Cell Biol 15, 6582-92. 3. Hafizi, S. and Dahlbäck, B. (2006) Cytokine Growth Factor Rev 17, 295-304. 4. Sather, S. et al. (2007) Blood 109, 1026-33. 5. Keating, A.K. et al. (2006) Oncogene 25, 6092-100.

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