antibody

MAP3K7 (Mitogen-activated Protein Kinase Kinase Kinase 7, Transforming Growth Factor-beta-activated Kinase 1, TGF-beta-activated Kinase 1, TAK1)

M2867-56S

100 ug

polyclonal

domestic goat

nonconjugated

human, mouse

MAP3K7 (Mitogen-activated Protein Kinase Kinase Kinase 7, Transforming Growth Factor-beta-activated Kinase 1, TGF-beta-activated Kinase 1, TAK1)

goat

Pab

Antibodies

100 ug

IgG

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signal transduction of TRAF6, various cytokines including interleukin-1 (IL-1), transforming growth factor-beta (TGFB), TGFB-related factors like BMP2 and BMP4, toll-like receptors (TLR), tumor necrosis factor receptor CD40 and B-cell receptor (BCR). Ceramides are also able to activate MAP3K7/TAK1. Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K1/MEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7. These MAP2Ks in turn activate p38 MAPKs, c-jun N-terminal kinases (JNKs) and I-kappa-B kinase complex (IKK). Both p38 MAPK and JNK pathways control the transcription factors activator protein-1 (AP-1), while nuclear factor-kappa B is activated by IKK. MAP3K7 activates also IKBKB and MAPK8/JNK1 in response to TRAF6 signaling and mediates BMP2-induced apoptosis. In osmotic stress signaling, plays a major role in the activation of MAPK8/JNK1, but not that of NF-kappa-B. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:8000 Western Blot: 1-2ug/ml, In transfected HEK293 transiently expressing full-length human MAP3K7 (myc and DYKDDDDK tagged) on ~75kD bands Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Mo Rt

Synthetic peptide corresponding to C-AELDQDEKDQQNT, from human MAP3K7, at the internal region of the protein (NP_003179.1, NP_663304.1).

Recognizes human MAP3K7. Species Crossreactivity: mouse. Species sequence homology: rat.

Purified by immunoaffinity chromatography

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

~0.5mg/ml

Product Type: Pab Host: goat Source: human Concentration: ~0.5mg/ml Form: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA. Purity: Purified by immunoaffinity chromatography. Immunogen: Synthetic peptide corresponding to C-AELDQDEKDQQNT, from human MAP3K7, at the internal region of the protein (NP_003179.1, NP_663304.1). Specificity: Recognizes human MAP3K7. Species Crossreactivity: mouse. Species sequence homology: rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E WB

-20°C

https://usbio-images.r.worldssl.net/prodimages/6/M2867-56S_1.jpg