MDM2 (Murine Double Minute-2) | US Biological M2800-03B product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

MDM2 (Murine Double Minute-2)

catalog :

M2800-03B

quantity :

200 ug

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

M2800-03B

Product wo Prefix :

MDM2 (Murine Double Minute-2)

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

200 ug

Isotype :

IgG

Desc1 :

Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 2-4ug/ml detects MDM2 in RIPA lysates from HeLa cells. Hela cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with M2800-03B (2ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Immunogen :

Synthetic peptide corresponding to amino acids 164-185 of human MDM2, CAISETEENSDELSGERQRKRHK (KLH) with an N-terminal cysteine added for conjugation purposes. The immunizing peptide is identical in both p90MDM2 and p76MDM2.

Specificity :

Recognizes human MDM2 at 76kD.

Purity :

Purified by Protein A affinity chromatography.

Form :

Supplied as a liquid in PBS, pH7.6, 0.07% sodium azide and 30% glycerol.

Concentration :

~0.5mg/ml

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: ~0.5mg/ml Form: Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%. Purity: Purified by Protein A affinity chromatography. Immunogen: KLH-conjugated, synthetic peptide (CAISETEENSDELSGERQRKRHK) corresponding to amino acids 164-185 of human MDM2 (mouse double minute 2) with an N-terminal cysteine added for conjugation purposes. The immunizing peptide is identical in both MDM2 isoforms, p90MDM2 and p76MDM2. Specificity: Recognizes MDM2, Mr 76kD. Species Crossreactivity: Human. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

Storage Temperature :

-20°C

Reference :

1.Léveillard, T. and B. Wasylyk, J. Biol. Chem. 272: 30651-30661, 1997.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/35/M2800-03B_1.jpg