Matrix Metalloproteinase 9 (MMP-9, Gelatinase B, Collagenase 4) | US Biological M2425-03 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Matrix Metalloproteinase 9 (MMP-9, Gelatinase B, Collagenase 4)

catalog :

M2425-03

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

M2425-03

Product wo Prefix :

Matrix Metalloproteinase 9 (MMP-9, Gelatinase B, Collagenase 4)

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

Matrix metalloproteinase (MMP) is a family of proteinases that target many extracellular proteins, such as other type of proteinases, growth factors, cell surface receptors and adhesion molecules (1). Among the family members, MMP-2, MMP-3, MMP-7 and MMP-9 have been characterized as important factors for normal tissue remodeling during embryo development and wound healing, tumor invasion, angiogenesis, carcinogenesis and apoptosis (2-4). MMP activity is correlated with cancer development (2). One way of MMP regulation is controlling the overall proteinase expression at the transcription step (5). Once synthesized, MMP exists in a latent proenzyme form. The maximum MMP activity requires proteolytic cleavage of MMPs to generate active MMPs by release of the inibitory propeptide domain from the whole molecule (5,6). Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunohistochemistry: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Immunogen :

Synthetic phosphopeptide corresponding to residues around 107-117 of human MMP-9.

Specificity :

Detects human full length (proenzyme, 92kD) and cleaved (active enzyme, 84kD) MMP-9.

Purity :

Purified by protein A and peptide affinity chromatography.

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by protein A and peptide affinity chromatography. Immunogen: Synthetic phosphopeptide corresponding to residues around 107-117 of human MMP-9. Specificity: Detects human full length (proenzyme, 92kD) and cleaved (active enzyme, 84kD) MMP-9. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

IHC WB

Storage Temperature :

-20°C

Reference :

(1) McCawley, L.J. and Matrisian, L.M. (2001) Curr. Opin. Cell Biol. 13, 534–540. (2) Coussens, L.M. et al. (2002) Science 295, 2387–2391. (3) Sternlicht, M.D. et al. (1999) Cell 98, 137–146. (4) Vu, T.H. et al. (1998) Cell 93, 411–422. (5) Nagase, H. et al. (1990) Biochemistry 29, 5783–5789. (6) Fang, K.C. et al. (1997) J. Biol. Chem. 272, 25628–25635.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/12/M2425-03_1.jpg

Picture4 Text :

Immunohistochemical analysis of paraffin-embedded human squamous cell carcinoma of the esophagus, using M2425-03.