Mast Cell Chymase (CMA1) | US Biological M2414-01B product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Mast Cell Chymase (CMA1)

catalog :

M2414-01B

quantity :

100 ug

clonality :

polyclonal

host :

domestic sheep

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

M2414-01B

Product wo Prefix :

Mast Cell Chymase (CMA1)

Host :

sheep

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ug

Isotype :

IgG

Desc1 :

Chymase (also mast cell protease I) is a secreted, 30kD glycoprotein member of the granzyme subfamily, peptidase S1 family of enzymes. It is secreted by mast cells and vascular smooth muscle cells, and converts angiotensin I into angiotensin II. This promotes fibroblast proliferation and secretion of ECM. Mature human chymase is 226aa in length. It contains one peptidase S1 domain (aa22-245). There is one potential alternate splice form. It shows a deletion of the 99 C-terminal amino acids (aa49-247). Applications: Suitable for use in Western Blot, Immunoprecipitation and ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml using lysate of human small intestine tissue Immunoprecipitation: 25ug/ml using rhChymase from conditioned media of transfected NS0 cells. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Immunogen :

Recombinant protein corresponding to Gly20-Asn247 from human Chymase expressed in NS0 cells. Swiss/UniProt Accession: P23946.

Specificity :

Recognizes human Chymase. Crossreactivity: ~10% with MCPT1 and <1% with rhGranzyme-B, rhGranzyme-H, rmMCPT6 and rmMCPT7.

Purity :

Purified by immunoaffinity chromatography

Form :

Supplied as a lyophilized powder from PBS, ph 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.

Concentration :

~0.2mg/ml (after reconstitution)

Desc2 :

Product Type: Pab Isotype: IgG Host: sheep Source: human Concentration: ~0.2mg/ml (after reconstitution) Form: Supplied as a lyophilized powder from PBS, ph 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS. Purity: Purified by immunoaffinity chromatography. Immunogen: Recombinant protein corresponding to aa20-247 from human Chymase expressed in NS0 cells. Swiss/UniProt Accession: P23946. Specificity: Recognizes human Chymase. Crossreactivity: ~10% with MCPT1 and <1% with rhGranzyme-B, rhGranzyme-H, rmMCPT6 and rmMCPT7. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IP WB

Storage Temperature :

-20°C

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/31/M2414-01B_1.jpg