antibody

MAP Kinase, p44/42 (Erk1/Erk2, Extracellular Signal Regulated Kinase)

M2360-02

200 ul

polyclonal

domestic rabbit

nonconjugated

MAP Kinase, p44/42 (Erk1/Erk2, Extracellular Signal Regulated Kinase)

rabbit

Pab

Antibodies

200 ul

IgG

Both p44 and p42 MAP kinases (Erk1 and Erk2) function in a protein kinase cascade that plays a critical role in the regulation of cell growth and differentiation. MAP kinases are activated by a wide variety of extracellular signals including growth and neurotrophic factors, cytokines, hormones and neurotransmitters. Activation of MAP kinases occurs through phosphorylation of threonine and tyrosine (202 and 204 of human MAP kinase [Erk1] or 183 and 185 of rat Erk2) at the sequence T*EY* by a single upstream MAP kinase kinase (MEK). Both kinases are known to weakly autophosphorylate on tyrosine. Applications: Suitable for use in Immunofluorescence, Immunocytochemistry, ELISA, Western Blot, Immunohistochemistry,Immunoprecipitation and Flow Cytometry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000; Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunohistochemistry (Paraffin): 1:100. Use citrate as unmasking buffer. Immunofluorescence (IC): 1:100 Flow Cytometry: 1:50 Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Bo Hm Hu Mk Mo Po Rt

EC=2.7.11.24

Synthetic peptide derived from the sequence in the C-terminus of rat p42 MAP kinase, coupled to KLH.

Recognizes endogenous levels of total rat p44/42 MAP kinase (Erk1/Erk2) protein. In some cell types, recognizes p44 MAPK more readily than p42 MAPK . Does not recognize JNK/SAPK nor p38 MAP kinase. Species Crossreactivity: human, mouse,hamster, zebrafish, monkey, porcine, bovine, mink and S. cerevisiae.

Purified by Protein A and peptide affinity chromatography.

Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

Not Determined

Product Type: Pab Isotype: IgG Host: rabbit Source: rat Concentration: Not Determined Form: Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide derived from the sequence in the C-terminus of rat p42 MAP kinase, coupled to KLH. Specificity: Recognizes endogenous levels of total rat p44/42 MAP kinase (Erk1/Erk2) protein. In some cell types, recognizes p44 MAPK more readily than p42 MAPK . Does not recognize JNK/SAPK nor p38 MAP kinase. Species Crossreactivity: human, mouse,hamster, zebrafish, monkey, porcine, bovine, mink and S. cerevisiae. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E FC IC IF IHC IP WB

-20°C

1. Marshall, C.J., Cell 80: 179-185 (1995). 2. Hunter, T., Cell 80: 225-236 (1995). 3. Hill, C.S. and Treisman, R., Cell 80: 199-211 (1995). 4. Cowley, S., et al., Cell 77: 841-852 (1994). 5. Sturgill, T. W., et al., Nature 334: 715-718 (1988). 6. Payne, D.M., et al., EMBO J. 10: 885-892 (1991). 7. Domina, A.M., et al., J. Biol. Chem. 275: 21,688-20,984 (2000). 8. Hayne, C., et al., J. Biol. Chem. 275: 31,876-31,882 (2000). 9. Tan, J., et al., J. Neurosci. 20: 7,587-7,594 (2000).

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