MAP Kinase, p38, phosphorylated (Thr180/Tyr182) (MapK14, Mitogen-activated Protein Kinase p38 alpha, Mitogen-activated Protein Kinase 14, CRK1, CSBP1, CSBP2) | US Biological M2352-05H product information

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product summary

company name :

US Biological

product type :

antibody

product name :

MAP Kinase, p38, phosphorylated (Thr180/Tyr182) (MapK14, Mitogen-activated Protein Kinase p38 alpha, Mitogen-activated Protein Kinase 14, CRK1, CSBP1, CSBP2)

catalog :

M2352-05H

quantity :

200 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human

product information

Catalog Number :

M2352-05H

Product wo Prefix :

MAP Kinase, p38, phosphorylated (Thr180/Tyr182) (MapK14, Mitogen-activated Protein Kinase p38 alpha, Mitogen-activated Protein Kinase 14, CRK1, CSBP1, CSBP2)

Host :

rabbit

Product Type :

Pab

Antigen Modification :

Phosphorylated

Category :

Antibodies

Size1 :

200 ul

Isotype :

IgG

Desc1 :

p38 MAP kinase (MAPK), also called RK or CSBP, is the mammalian orthologue of the yeast HOG kinase which participates in a signaling cascade controlling cellular responses to cytokines and stress. Four isoforms of p38 MAP kinase, p38a, b and g (also known as ERK6 or SAPK3) and delta (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAP kinase is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharides LPS), UV light and growth factors. MKK3, MKK6 and SEK activate p38 MAP kinase by phosphorylation at Thr180 and Tyr182. Activated p38 MAP kinase has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2, Max and MEF2. Applications: Suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000. Iincubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20. Immunohistochemistry (Paraffin): 1:50. Antigen retrieval: heating in citrate buffer. Dilute antibody in TBS-Tween plus 5% normal goat serum. Immunofluorescence (IF-IC): 1:100 Optimal dilution determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Dr Hu Mk Mo Rt Ze

Alternate Name :

EC=2.7.11.24

Immunogen :

Synthetic phosphopeptide corresponding to residues surrounding Thr180/Tyr182 of human p38 MAPK. Species Sequence Homology: mink, zebrafish and hamster.

Specificity :

Recognizes endogenous levels of human p38 MAPK only when dually phosphorylated at threonine 180 and tyrosine182. Does not crossreact with the phosphorylated forms of either p42/44 MAPK or SAPK/JNK. Species Crossreactivity: monkey, mouse, rat and D. melanogaster.

Purity :

Ascites

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 01mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Concentration :

Not Determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not Determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 01mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Ascites Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Thr180/Tyr182 of human p38 MAPK. Species Sequence Homology: mink, zebrafish and hamster. Specificity: Recognizes endogenous levels of human p38 MAPK only when dually phosphorylated at threonine 180 and tyrosine182. Does not crossreact with the phosphorylated forms of either p42/44 MAPK or SAPK/JNK. Species Crossreactivity: monkey, mouse, rat and D. melanogaster. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

IF IHC WB

Storage Temperature :

-20°C

Reference :

1. Rouse, J. et al. (1994) Cell 78; 1027-1037. 2. Han, J. et al. (1994) Science 265; 808-811.3. Lee, J.C. et al. (1994) Nature 372; 739-746. 4. Freshney, N.W. et al. (1994) Cell 78; 1039-1049. 5. Raingeaud, J. et al. (1995) J. Biol. Chem. 270;7420-7426. 6. Zervos, A.S. et al. (1995) Proc. Natl. Acad. Sci. USA 92;10531-10534. 7. Zhao, M. et al. (1999) Mol. Cell. Biol. 19; 21-30. 8. Yang, S.H. et al. (1999) Mol. Cell. Biol. 19; 4028-4038.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/20/M2352-05H_1.jpg

Picture4 Text :

Immunohistochemistry of paraffin-embedded NIH/3T3 cells, untreated (left) or anisomycin-treated (right), using M2352-05H.

Picture 5 File Name :

https://usbio-images.r.worldssl.net/prodimages/20/M2352-05H_5.jpg

Picture5 Text :

Confocal immunofluorescent analysis of HeLa cells -/+ UV light, labeled with M2352-05H (green). Absence of staining in untreated cells (left) and nuclear localization in treated cells (right). Red = Actin filaments (phalloidin).