Macrophage Inflammatory Protein 1 beta (MIP-1b, CCL4) | US Biological M1202-31B product information

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product summary

company name :

US Biological

product type :

antibody

product name :

Macrophage Inflammatory Protein 1 beta (MIP-1b, CCL4)

catalog :

M1202-31B

quantity :

100 ug

clonality :

polyclonal

host :

domestic goat

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

M1202-31B

Product wo Prefix :

Macrophage Inflammatory Protein 1 beta (MIP-1b, CCL4)

Host :

goat

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ug

Isotype :

IgG

Desc1 :

Applications: Suitable for use in neutralization of bioactivity, ELISA, Western Blot, and Immunohistochemistry. Other applications not tested. Recommended Dilution: Neutralization: 0.3-1.5ug/ml in the presence of 0.04ug/ml of rhMIP-1b, using the BaF/3 hCCR5 transfected cell chemotaxis assay. In chemotaxis assays, this antibody will neutralize the biological activity of human MIP-1b and mouse MIP-1b at a 25-fold higher IgG concentration. Will not neutralize the biological activity of mouse MIP1b. ELISA: 0.5-1ug/ml with the appropriate secondary reagents to detect human MIP-1b. The detection limit is ~0.3ng/well. Western Blot: 0.1-0.2ug/ml. The detection limit is 1.5-3.0ng/lane, under non-reducing and reducing conditions. Immunohistochemistry: 0.5- 5ug/ml in cultured cells or tissue. sections. Optimal dilutions to be determined by the researcher. Endotoxin: <0.1EU/ml Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Country of Origin: USA

Calc Crossreactivity :

Hu Mo

Immunogen :

Recombinant protein corresponding to human Macrophage Inflammatory Protein 1 beta (MIP1 beta, CCL4), Sf 21-derived.

Specificity :

Recognizes Macrophage Inflammatory Protein 1 beta (MIP1 beta). based on ELISA and Western Blot data, <10% crossreactivity with mouse MIP-1b, human MIP-1b, human MCP-2 and <5% crossreactivity with mouse MIP-1b. <1% crossreactivity with other chemokines tested.

Purity :

Purified by immunoaffinity chromatography

Form :

Supplied as a liquid in PBS, pH 7.2.

Concentration :

~0.1mg/ml

Desc2 :

Product Type: Pab Isotype: IgG Host: goat Source: human Concentration: ~0.1mg/ml Form: Supplied as a liquid in PBS, pH 7.2. Purity: Purified by immunoaffinity chromatography. Immunogen: Recombinant protein corresponding to human Macrophage Inflammatory Protein 1 beta (MIP1 beta, CCL4), Sf 21-derived. Specificity: Recognizes Macrophage Inflammatory Protein 1 beta (MIP1 beta). based on ELISA and Western Blot data, <10% crossreactivity with mouse MIP-1b, human MIP-1b, human MCP-2 and <5% crossreactivity with mouse MIP-1b. <1% crossreactivity with other chemokines tested. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IHC N WB

Storage Temperature :

-20°C

Reference :

Megjugorac, N.J. et al., (2003) J. Leukocyte Biology 75:504-514.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/30/M1202-31B_1.jpg