antibody

Lymphoid Enhancer Factor 1 (LEF1, Lymphoid Enhancer-binding Factor 1, HGNC:6551, T cell Specific Transcription Factor 1 alpha, TCF1 alpha)

L8005-10

100 ul

monoclonal

domestic rabbit

nonconjugated

7H38(C12A5)

Lymphoid Enhancer Factor 1 (LEF1, Lymphoid Enhancer-binding Factor 1, HGNC:6551, T cell Specific Transcription Factor 1 alpha, TCF1 alpha)

rabbit

Mab

Antibodies

100 ul

7H38(C12A5)

IgG

LEF1/TCF are members of the high mobility group (HMG) DNA binding protein family of transcription factors which consists of four members: Lymphoid enhancer factor 1 (LEF1), T Cell Factor 1 (TCF1), TCF3 and TCF4 (1). LEF1 and TCF1 were originally identified as important factors regulating early lymphoid development (2) and are downstream factors in the Wnt signaling pathway. LEF1/TCF bind to Wnt response elements to provide a docking site for beta-catenin which translocates to the nucleus and promotes the transcription of target genes upon activation of Wnt signaling (3). LEF1/TCF proteins are dynamically expressed during development and aberrant activation of the Wnt signaling pathway is involved in many types of cancers such as colon cancers (4,5). LEF1 has several isoforms due to alternative splicing. LEF1 also has an alternative promoter that is preferentially active in lymphocytes. The isoforms generated by this alternative promoter have no amino-terminal beta-catenin binding domain and may function in a dominant negative manner (6-8). Applications: Suitable for use in Western Blot, Flow Cytometry, Immunofluorescence. Other applications not tested. Recommended Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in 1X TBS, 5% BSA, 0.1% Tween-20 at 4ºC with gentle shaking, overnight. Flow Cytometry: 1:400 Immunofluorescence (IF-IC): 1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Mo Rt

Synthetic peptide corresponding to residues surrounding Pro82 of human LEF1.

Recognizes endogenous level of total human LEF1 protein at ~25-58kD. Does not recognize the dominant negative forms of LEF1 generated by an alternative promoter. Species Crossreactivity: Mouse, rat.

Purified

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Not determined

Product Type: Mab Isotype: IgG Clone No: 7H38(C12A5) Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Synthetic peptide corresponding to residues surrounding Pro82 of human LEF1. Specificity: Recognizes endogenous level of total human LEF1 protein at ~25-58kD. Does not recognize the dominant negative forms of LEF1 generated by an alternative promoter. Species Crossreactivity: Mouse, rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

FC IF WB

-20°C

1. Waterman, M.L., Cancer Metastasis Rev. 23: 41-52 (2004). 2. Schilham, M.W. & Clevers, H., Semin. Immunol. 10: 127-132 (1998). 3. Brantjes, H., et al., Biol. Chem. 383: 255-261 (2002). 4. Reya, T. & Clevers, H., Nature 434: 843-850 (2005). 5. Logan, C.Y. & Nusse, R., Annu. Rev. Cell Dev. Biol. 20: 781-810 (2004). 6. Hovanes, K., et al., Nucleic Acids Res. 28: 1994-2003 (2000). 7. Hovanes, K., et al., Nat. Genet. 28: 53-57 (2001). 8. Kobielak, A., et al., Acta. Biochim. Pol. 48: 221-226 (2001).

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