Luciferase | US Biological L5999-27 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Luciferase

catalog :

L5999-27

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

6A106

product information

Catalog Number :

L5999-27

Product wo Prefix :

Luciferase

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ug

Clone # USB :

6A106

Isotype :

IgG1

Desc1 :

Luciferase is a luminescent enzyme produced by Photinus pyralis, also referred to as the common eastern firefly or North American firefly. It is known for its role as a reporter protein and can be used to quantify expression levels from promoters in tissue culture and in vivo. Luciferase catalyses the oxygenation of luciferin using ATP and oxygen to produce oxyluciferin, a highly unstable, singlet-excited compound that emits light upon relaxation to its ground state. Bioluminescence assay systems have become increasingly used in biology and medical research laboratories in addition to (or as alternatives to) fluorescence and chemiluminescence detection strategies. Luciferase enzymes isolated from different animal species have inherent variability in light emission, allowing two or more luciferase enzymes to be used in combination for multiplex analyses, including in vivo imaging, cell viability and single and dual-spectral luciferase reporter assays. Applications: Suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence. Other applications have not been tested. Recommended Dilutions: Western Blot: 1-3ug/ml. Observed in mouse heart, liver, kidney, and cortex tissues containing added purified luciferase protein. Immunohistochemistry (Frozen): 15-50ug/ml. Detects tissue specific expression of Luciferase protein in Drosophila embryos using Gal4-UAS system with two different Gal4 drivers. Immunofluorescence: 10-30ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Immunogen :

Luciferase protein from Photinus pyralis

Specificity :

Recognizes Luciferase. No cross reactivity to mouse heart, NIH3T3, Jurkat, and human fibroblasts.

Purity :

Purified by Protein A affinity chromatography.

Form :

Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.

Concentration :

~0.5mg/ml

Desc2 :

Product Type: Mab Isotype: IgG1 Clone No: 6A106 Host: mouse Source: human Concentration: ~0.5mg/ml Form: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide. Purity: Purified by Protein A affinity chromatography. Immunogen: Luciferase protein from Photinus pyralis Specificity: Recognizes Luciferase. No cross reactivity to mouse heart, NIH3T3, Jurkat, and human fibroblasts. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

IF IHC WB

Storage Temperature :

-20°C

Reference :

1. Tatsumi H. etal. (1996) Anal Biochem 243(1):176-80. 2. Van Leeuwen W. et al. (2000) Plant Mol. Biol. Reporter 18(2):143. 3. Chem B. et al. (1994) J. Virol 68(2): 54-60. 4. Kobatake E. et al. (1993) Anal Biochem 208(2):300-5. 5. Park S. et al. (1992) J. Gen Microbiol 138(12):2619-27. 6. Nicolas M. et al. (1989) 37(5):663-74.