antibody

LSD1 (Lysine-Specific Histone Demethylase, Amine Oxidase Flavin-containing Domain Protein 2, AOF2)

L4595-50C

100 ul

polyclonal

domestic rabbit

nonconjugated

LSD1 (Lysine-Specific Histone Demethylase, Amine Oxidase Flavin-containing Domain Protein 2, AOF2)

rabbit

Pab

Antibodies

100 ul

IgG

Lysine-specific demethylase 1 (LSD1; also known as AOF2 and BHC110) is a nuclear homolog of amine oxidases and functions as a histone demethylase and transcription cofactor (1). Gene activation and repression is specifically regulated by the methylation state of distinct lysine residues in histone proteins. For example, methylation of histone H3 Lys4 facilitates transcriptional activation by coordinating the recruitment of BPTF, a component of the NURF chromatin remodeling complex, and WDR5, a component of multiple histone methyltransferase complexes (2,3). In contrast, methylation of histone H3 (Lys9) facilitates transcriptional repression by recruiting HP1 (4,5). LSD1 is a component of the CoREST transcriptional co-repressor complex, which contains CoREST, CtBP, HDAC1 and HDAC2. As part of this complex, LSD1 demethylates mono-methyl and di-methyl histone H3 Lys4 via an FADdependent oxidation reaction, and facilitates the repression of neuronal-specific genes in non-neuronal cells (1,6,7). In contrast, when LSD1 is associated with androgen-receptor in human prostate cells, it demethylates mono-methyl and di-methyl histone H3 Lys9 and facilitates androgen-receptordependent transcriptional activation (8). Therefore, LSD1 can function as a co-repressor or co-activator, depending on gene context. LSD1 activity is inhibited by the amine oxidase inhibitors pargyline, deprenyl, clorgyline and tranylcypromine (8). Applications: Suitable for use in Immunofluorescence/Immunocytochemistry, Flow Cytometry, ChIP, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:200 IHC Protocol: Citrate/TBST Immunofluorescence (IC): 1:400 Chromatin IP: 1:50 Flow Cytometry: 1:400 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Mk Mo Rt

Synthetic peptide corresponding to the C-terminus of human LSD.

Recognizes endogenous levels of total human LSD1 protein. Species crossreactivity: mouse, rat and monkey.

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA and 50% glycerol.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA and 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to the C-terminus of human LSD. Specificity: Recognizes endogenous levels of total human LSD1 protein. Species crossreactivity: mouse, rat and monkey. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E FC IF IHC IP WB

-20°C

1. Shi, Y. et al. (2004) Cell 119:941–953. 2. Wysocka, J. et al. (2006) Nature 442:86–90. 3. Wysocka, J. et al. (2005) Cell 121:859–872. 4. Jacobs, S.A. and Khorasanizadeh, S. (2002) Science 295:2080–2083. 5. Nielsen, P.R. et al. (2002) Nature 416:103–107. 6. Shi, Y.J. et al. (2005) Mol. Cell 19:857–864. 7. Lee, M.G. et al. (2005) Nature 437:432–435. 8. Metzger, E. et al. (2005) Nature 437:436–439.

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