antibody

LKB 1 (STK11, Serine/Threonine Kinase 11, LKB1, PJS, Renal carcinoma antigen NY-REN-19, Serine/threonine-protein kinase 11, Serine/threonine-protein kinase LKB1)

L3185-05

100 ul

monoclonal

domestic rabbit

nonconjugated

7H36(27D10)

LKB 1 (STK11, Serine/Threonine Kinase 11, LKB1, PJS, Renal carcinoma antigen NY-REN-19, Serine/threonine-protein kinase 11, Serine/threonine-protein kinase LKB1)

rabbit

Mab

Antibodies

100 ul

7H36(27D10)

IgG

LKB1 (STK11) is a serine/threonine kinase and tumor suppressor that helps control cell structure, apoptosis and energy homeostasis through regulation of numerous downstream kinases. A cytosolic protein complex comprised of LKB1, putative kinase STRAD, and the MO25 scaffold protein, activates both AMP-activated protein kinase (AMPK) and several AMPK-related kinases. AMPK plays a predominant role as the master regulator of cellular energy homeostasis, controlling downstream effectors that regulate cell growth and apoptosis in response to cellular ATP concentrations. LKB1 appears to be phosphorylated in cells at several sites, including human LKB1 at Ser31/325/428 and Thr189/336/363. Mutation in the corresponding LKB1 gene causes Peutz-Jeghers syndrome (PJS), an autosomal dominant disorder characterized by benign GI tract polyps and dark skin lesions of the mouth, hands and feet. A variety of other LKB1 gene mutations have been associated with the formation of sporadic cancers in several tissues. Applications: Suitable for use in Immunoprecipitation and Western Blot. Other applications have not been tested. Recommended Dilutions: Immunoprecipitation: 1:100 Western Blot: 1:1000. Incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Positive Control: Caki-1, COS, and insulin treated 293T cell extracts. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Mk

Synthetic peptide derived from the sequence of human LKB1.

Recognizes endogenous levels of human LKB1 protein at ~54kD. Species Crossreactivity: monkey

Purified

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Not Determined

Product Type: Mab Isotype: IgG Clone No: 7H36(27D10) Host: rabbit Source: human Concentration: Not Determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Synthetic peptide derived from the sequence of human LKB1. Specificity: Recognizes endogenous levels of human LKB1 protein at ~54kD. Species Crossreactivity: monkey Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

IP WB

-20°C

1. Baas, A.F., et al., Trends Cell Biol. 14: 312-319 (2004). 2. Marignani, P.A., J. Clin. Pathol. 58: 15-19 (2005). 3. Lizcano, J.M., et al., EMBO J. 23: 833-843 (2004). 4. Hardie, D.G., J. Cell Sci. 117: 5479-5487 (2004). 5. Sapkota, G.P., et al., Biochem. J. 362: 481-490 (2002). 6. Jenne, D.E., et al., Nat. Genet. 18: 38-43 (1998). 7. Sanchez-Cespedes, M., Oncogene 26: 7825-7832 (2007).

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