antibody

LIR-8 (Leukocyte Immunoglobulin-like Receptor 8, CD85c)

L2637-45

100 ug

polyclonal

domestic goat

nonconjugated

LIR-8 (Leukocyte Immunoglobulin-like Receptor 8, CD85c)

goat

Pab

Antibodies

100 ug

IgG

Human LIR-8 (also known as CD85c and LILRB5) is a 587 amino acid transmembrane protein of the family of Leukocyte Immunoglobin-like Receptors. LIR-8 has a long N-terminus extracellular domain with four C2-type lg-like domains, and a short cytoplasmic tail with two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) typically involved in modulation of cellular responses. Human LIR-8 is expressed in natural killer (NK) cells. There are at least two other known isoforms, one with 53 aa longer cytoplasmic domain, the other with a 100 aa deletion affecting one of the lg-like extracellular domains. Applications: Suitable for use in Direct ELISA, Western Blot and Flow Cytometry. Other applications not tested. Recommended Dilutions: Western Blot: 0.1ug/ml Flow Cytometry: 2.5ug/10e5 cells. Validated using human peripheral blood monocytes and granulocytes. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu

Recombinant protein corresponding to aa18-456 from human LIR-8, expressed in NS0 cells (O75023).

Recognizes human LIR-8, Shows <20% crossreactivity with rhLIR-4, ~10% with rhILT5, rhLIR-5, rhLIR-6, and rhLILRA5 and <5% with rhILT2, rhILT4, and rhLIR-7.

Purified by immunoaffinity chromatography

Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.

~0.2mg/ml (after reconstitution)

Product Type: Pab Isotype: IgG Host: goat Source: human Concentration: ~0.2mg/ml (after reconstitution) Form: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS. Purity: Purified by immunoaffinity chromatography Immunogen: Recombinant protein corresponding to aa18-456 from human LIR-8, expressed in NS0 cells (O75023). Specificity: Recognizes human LIR-8, Shows <20% crossreactivity with rhLIR-4, ~10% with rhILT5, rhLIR-5, rhLIR-6, and rhLILRA5 and <5% with rhILT2, rhILT4, and rhLIR-7. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E FC WB

-20°C