LIM Kinase 1 | US Biological L2212 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

LIM Kinase 1

catalog :

L2212

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

L2212

Product wo Prefix :

LIM Kinase 1

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

LIM kinases (LIMK1 and LIMK2) are serine/ threonine kinases that have two zinc finger motifs, known as LIM motifs, in their N-terminal regulatory domain (1). LIM kinases are involved in actin cytoskeleton regulation through Rho-family GTPases and downstream kinases PAKs and ROCK (2,3). PAK1 and ROCK phosphorylate LIMK1 or LIMK2 at the conserved Thr508 or Thr505 in the activation loop, which increases the activity of the kinases (3-5). Activated LIM kinases inhibit the actin depolymerization activity of cofilin by phosphorylation at the N-terminal serine 3 residue of cofilin (6,7). Applications: Suitable for use in Western Blot, Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Hu Mk Mo Rt

Immunogen :

Synthetic peptide corresponding to the C-terminal residues of human LIMK1.

Specificity :

Recognizes endogenous levels of total human LIMK1 protein independent of phosphorylation. Does not crossreact with LIMK2. Species Crossreactivity: mouse, rat and monkey.

Purity :

Purified by Protein A and peptide affinity chromatography.

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to the C-terminal residues of human LIMK1. Specificity: Recognizes endogenous levels of total human LIMK1 protein independent of phosphorylation. Does not crossreact with LIMK2. Species Crossreactivity: mouse, rat and monkey. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

IP WB

Storage Temperature :

-20°C

Reference :

1. Okano, I. et al. (1995) J. Biol. Chem. 270:31321–31330. 2. Maekawa, M. et al. (1999) Science 285:895–898. 3. Edwards, D.C. et al. (1999) Nat. Cell Biol. 1:253–259. 4. Ohashi, K. et al. (2000) J. Biol. Chem. 275:3577–3582. 5. Sumi, T. et al. (2001) J. Biol. Chem. 276:670–676. 6. Arber, S. et al. (1998) Nature 393:805–809. 7. Yang, N. et al. (1998) Nature 393:809–812.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/22/L2212_1.jpg