antibody

Lactate dehydrogenase A (LDHA)

L1012

100 ul

polyclonal

domestic rabbit

nonconjugated

Lactate dehydrogenase A (LDHA)

rabbit

Pab

Antibodies

100 ul

IgG

Lactate dehydrogenase (LDH) catalyzes the interconversion of pyruvate and NADH to lactate and NAD+. When the oxygen supply is too low for mitochondrial ATP production, this reaction recycles NADH generated in glycolysis to NAD+, which refeeds into glycolysis. In muscle cells, the major form of LDH is the isozyme A (LDHA). The LDHA promoter contains HIF-1-alpha binding sites (1). Under hypoxic conditions, the expression of LDHA is induced (2). During intensive and prolonged muscle exercise, lactate accumulates in muscle cells when the supply of oxygen does not meet demand. When oxygen levels return to normal, LDH converts lactate to pyruvate to generate ATP in the mitochondrial electron transport chain. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu Mk Mo Rt

Synthetic peptide corresponding to the sequence of human LDHA.

Detects endogenous levels of total human LDHA. Species Crossreactivity: mouse, monkey and rat

Purified by peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

As reported

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: As reported Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to the sequence of human LDHA. Specificity: Detects endogenous levels of total human LDHA. Species Crossreactivity: mouse, monkey and rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

WB

-20°C

(1) Semenza, G.L. et al. (1996) J Biol Chem 271, 32529–37. (2) Semenza, G.L. (2007) Biochem J 405, 1–9.

https://usbio-images.r.worldssl.net/prodimages/14/L1012_1.jpg