KIR2DL4 (p58 KIR, CD158, KIR-103AS, Killer Cell Immunoglobulin (Ig)-like Receptor) (APC) discontinued | US Biological K1858-49B product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

KIR2DL4 (p58 KIR, CD158, KIR-103AS, Killer Cell Immunoglobulin (Ig)-like Receptor) (APC) discontinued

catalog :

K1858-49B

quantity :

100Tests

clonality :

monoclonal

host :

mouse

conjugate :

APC

clone name :

8B31

reactivity :

human

product information

Catalog Number :

K1858-49B

Product wo Prefix :

KIR2DL4 (p58 KIR, CD158, KIR-103AS, Killer Cell Immunoglobulin (Ig)-like Receptor) (APC) discontinued

Host :

mouse

Product Type :

Mab

Conjugate :

APC

Category :

Antibodies

Size1 :

100Tests

Clone # USB :

8B31

Isotype :

IgG2a

Desc1 :

KIR2DL4 is a type I transmembrane protein of the killer cell Ig-like receptor (KIR) family expressed on NK and subsets of gamma delta T and memory/effector abT cells. KIR2DL4 is a unique KIR; alleles are not clonally restricted but are expressed codominantly in all activated NK cells and constitutively on CD56hi NK cells. KIR members with two Ig-like domains (2D) usually express domains D1 and D2, but KIR2DL4 expresses D0 and D2. Other long-tailed (L) KIR have two cytoplasmic inhibitory signaling domains (ITIM), but KIR2DL4 has one ITIM and also exhibits characteristics of activating KIR. An arginine within the transmembrane sequence of KIR2DL4 interacts with the signaling molecule FcepsilonRI-gamma, while in activating KIR, a transmembrane lysine interacts with DAP12. The KIR2DL4 gene is highly polymorphic. Seven splice variants missing one or more exons have been identified, but it is not clear whether these are expressed. Several of the nine alleles identified encode a frameshift creating a prematurely truncated protein; it is estimated that up to 25% of humans do not express KIR2DL4 capable of reaching the cell surface. Human KIR2DL4 is 65%-83% amino acid identical to other primates. KIR receptors have no structural orthologs in non-primates, although mouse Ly49 proteins are functional orthologs. Cross-linking of KIR2DL4 induces NK cells to produce IFN-gamma; stimulation with IL-2 upregulates cell surface expression on CD56dim cells and allows cytotoxicity. Although a role in immune privilege of the fetus has been suggested due to reported recognition of fetal trophoblast HLA-G by KIR2DL4 in the maternal decidua, subsequent data have not supported this recognition. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: Neat; 10ul labels 1x10e5 cells. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Freezing Allophycocyanin conjugates will result in a substantial loss of enzymatic activity.

Calc Crossreactivity :

Immunogen :

Human KIR2DL4-transfected BaF/3 cells.

Specificity :

Recognizes human KIR2DL4.

Purity :

Purified by Protein G affinity chromatography.

Form :

Supplied as a liquid in PBS, 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).

Concentration :

Not Determined

Desc2 :

Product Type: Mab Isotype: IgG2a Clone No: 8B31 Host: mouse Source: human Concentration: Not Determined Form: Supplied as a liquid in PBS, 0.5% BSA, 0.1% sodium azide. Conjugated to Allophycocyanin (APC). Purity: Purified by Protein G affinity chromatography. Immunogen: Human KIR2DL4-transfected BaF/3 cells. Specificity: Recognizes human KIR2DL4. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

Storage Temperature :

4°C Do Not Freeze

Reference :

1. Lanier, L.L., Annu. Rev. Immunol. 23: 225-274 (2005). 2. Faure, M. and Long, E.O., J. Immunol. 168: 6208-6214 (2002). 3. Selvakumar, A., et al., Tissue Antigens 48: 285-294 (1996). 4. Chan, H.-W., et al., J. Exp. Med. 197: 245-255 (2003). 5. Kikuchi-Maki, A., et al., J. Immunol. 174: 3859-3863 (2005). 6. Rajagopalan, S., et al., J. Immunol. 167: 1877-1881 (2001). 7. Kikuchi-Maki, A., et al., J. Immunol. 171: 3415-3425 (2003). 8. Gedil, M.A., et al., Tissue Antigens 65: 402-418 (2005). 9. Witt, C.S., et al., Eur. J. Immunol. 32: 18-29 (2002). 10. Goodridge, J.P., et al., J. Immunol. 171: 1768-1774 (2003). 11. Ponte, M., et al., Proc. Natl. Acad. Sci. USA 96: 5674-5679 (1999).

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/17/K1858-49B_1.jpg