antibody

KPNA6 (Importin Subunit alpha-7, Karyopherin Subunit alpha-6, IPOA7)

K0102-11C

100 ug

polyclonal

domestic goat

nonconjugated

human, mouse, rat

KPNA6 (Importin Subunit alpha-7, Karyopherin Subunit alpha-6, IPOA7)

goat

Pab

Antibodies

100 ug

IgG

Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:128,000 Western Blot: 0.1-0.3ug/ml, observed in human skeletal muscle lysates on ~60kD bands Immunohistochemistry (Paraffin): 3-6ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Bo Ca Hu Mo Po Rt

Synthetic peptide corresponding to C-STTGESVITREMVE, from human KPNA6, at the internal of the protein (NP_036448.1).

Recognizes human KPNA6. Species sequence homology: Bovine, canine, mouse, porcine and rat.

Purified by immunoaffinity chromatography

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

~0.5mg/ml

Product Type: Pab Host: goat Source: human Concentration: ~0.5mg/ml Form: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA. Purity: Purified by immunoaffinity chromatography. Immunogen: Synthetic peptide corresponding to C-STTGESVITREMVE, from human KPNA6, at the internal of the protein (NP_036448.1). Specificity: Recognizes human KPNA6. Species sequence homology: Bovine, canine, mouse, porcine and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E IHC WB

-20°C

1. Ma J, Cao X. Regulation of Stat3 nuclear import by importin alpha5 and importin alpha7 via two different functional sequence elements. Cell Signal. 2006 Aug;18(8):1117-26. Epub 2005 Nov 18.

https://usbio-images.r.worldssl.net/prodimages/18/K0102-11C_1.jpg