JBP1 Methyltransferase (Skb1Hs) | US Biological J1100 product information

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product summary

company name :

US Biological

product type :

antibody

product name :

JBP1 Methyltransferase (Skb1Hs)

catalog :

J1100

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

J1100

Product wo Prefix :

JBP1 Methyltransferase (Skb1Hs)

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

Human Skb1Hs methyltransferase (also called JBP1), a homologue of yeast protein Skb1 and Hsl7p (1,2), is composed of 637 amino acid residues and contains motifs conserved among protein methyltransferases. It methylates histones and MBP in vitro (2). Yeast Hsl7p is involved in regulation of cell cycle progression through G2 by negatively regulating Swe1p, a protein tyrosine kinase that phosphorylates and inhibits Cdc28p (3). An Hsl7p homologue, Skb1, was identified in fission yeast by virtue of its yeast twohybrid interaction with Shk1p, a p21 (cdc42p/Rac) activated kinase (PAK) (4). Both proteins belong to the protein methyltransferase superfamily (5). Interestingly, human Skb1Hs methyltransferase was shown to interact with Jak kinases. This suggests the possibility that the Skb1Hs methyltransferase could link Jak to a PAK signaling pathway in mammalian cells. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications have not been tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Hu Mk Mo Rt

Immunogen :

Synthetic peptide corresponding to residues surrounding 100aa of human JBP1 Methyltransferase (KLH). MW: 70kD.

Specificity :

Recognizes endogenous levels of total human Skb1Hs methyltransferase protein at 70kD. Does not crossreact with other related mammalian methyltransferases. Species Crossreactivity: monkey, mouse and rat.

Purity :

Purified by Protein A and peptide affinity chromatography.

Form :

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA and 50% glycerol. No preservative added.

Concentration :

Not determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined. Form: Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA and 50% glycerol. No preservative added. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to residues surrounding 100aa of human JBP1 Methyltransferase (KLH). MW: 70kD. Specificity: Recognizes endogenous levels of total human Skb1Hs methyltransferase protein at 70kD. Does not crossreact with other related mammalian methyltransferases. Species Crossreactivity: monkey, mouse and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

IP WB

Storage Temperature :

-20°C

Reference :

1. Gilbreth, M. et al. (1998) Proc. Natl. Acad. Sci. USA 95, 14781–14786.2. Pollack, B.P. et al. (1999) J. Biol. Chem. 274, 31531–31542.3. Ma, X.J. et al. (1996) Genes Dev. 10, 1327–1340.4. Gilbreth, M. et al. (1996) Proc. Natl. Acad. Sci. USA 93, 13802–13807.5. Ma, X.J. et al. (2000) Trends Biochem. Sci. 25, 11–12.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/1/J1100_1.jpg