antibody

Jak3, phosphorylated (Tyr980/981) (Janus Kinase 3, EC 2.7.10.2, JAKL, Leukocyte Janus Kinase, LJAK)

J0902-06J

20 ul

monoclonal

domestic rabbit

nonconjugated

phosphorylated

10g85(D44E3)

Jak3, phosphorylated (Tyr980/981) (Janus Kinase 3, EC 2.7.10.2, JAKL, Leukocyte Janus Kinase, LJAK)

rabbit

Mab

Phosphorylated

Antibodies

20 ul

10g85(D44E3)

IgG

Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3 and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4) and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains. Jak3 is primarily expressed in hematopoietic cells and is required for immune cell function and development (6-8). It binds to the common gamma subunit (gc) and a shared receptor subunit also used by several cytokines including IL-2, IL-4, IL-7, IL-9, and IL-15 (9). IL-2 signaling and Stat5 activation is highly impaired by the loss of Jak3 (10,11). Jak3 is phosphorylated at multiple sites, including Tyr980 and 981 within its activation loop (12-14). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Mo

Synthetic phosphopeptide corresponding to a region surrounding Tyr980/981 of human and mouse Jak3 protein. Species Sequence Homology: Monkey (100%)

Recognizes endogenous levels of Jak3 when phosphorylated at Tyr980/981. Some reactivity may exist with single phosphorylation at these sites. Species crossreactivity: Human and mouse

Purified

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Not determined

Product Type: Mab Isotype: IgG Clone No: 10g85(D44E3) Host: rabbit Source: human Concentration: Not determined. Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Synthetic phosphopeptide corresponding to a region surrounding Tyr980/981 of human and mouse Jak3 protein. Species Sequence Homology: Monkey (100%) Specificity: Recognizes endogenous levels of Jak3 when phosphorylated at Tyr980/981. Some reactivity may exist with single phosphorylation at these sites. Species crossreactivity: Human and mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

WB

-20°C

https://usbio-images.r.worldssl.net/prodimages/37/J0902-06J_1.jpg