JAK1, phosphorylated (Tyr1022,1023) (Janus Kinase 1) | US Biological J0900-20A product information

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product summary

company name :

US Biological

product type :

antibody

product name :

JAK1, phosphorylated (Tyr1022,1023) (Janus Kinase 1)

catalog :

J0900-20A

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human

product information

Catalog Number :

J0900-20A

Product wo Prefix :

JAK1, phosphorylated (Tyr1022,1023) (Janus Kinase 1)

Host :

rabbit

Product Type :

Pab

Antigen Modification :

Phosphorylated

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

The Janus family tyrosine kinases, consisting of Jak1, Jak2, Jak3 and Tyk2, associate with a variety of cytokine receptors and are activated by ligand binding to these receptors (1). Upon ligand binding to cytokine receptors, Jaks phosphorylate themselves and their associated receptors, thereby providing multiple binding sites for signaling proteins containing SH2 or other phospho-tyrosine-binding domains, including Stats (2), Shc (3), insulin receptor substrates (4) and focal adhesion kinase (5). The tyrosine residues 1022/1023 of Jak1 in the putative activation loop are the homologous tyrosine residues 1054/1055 in Tyk2, which are important in the regulation of Tyk2 kinase activity (6). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Hu Mo

Immunogen :

Synthetic phosphopeptide corresponding to residues around Tyr1022/1023 of human Jak1. Species sequence homology: rat and bovine. Swiss/UniProt Accession: Q4LDX3

Specificity :

Recognizes endogenous levels of human Jak1 only when phosphorylated at tyrosines 1022/1023. Species Crossreactivity: mouse

Purity :

Purified by Protein A and peptide affinity chromatography.

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Also available without BSA & Azide. See J0900-20A1.

Concentration :

Not determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Also available without BSA & Azide. See J0900-20A1. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic phosphopeptide corresponding to residues around Tyr1022/1023 of human Jak1. Species sequence homology: rat and bovine. Swiss/UniProt Accession: Q4LDX3 Specificity: Recognizes endogenous levels of human Jak1 only when phosphorylated at tyrosines 1022/1023. Species Crossreactivity: mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

Storage Temperature :

-20°C

Reference :

(1) Leonard, W.J. and O’Shea, J.J. (1998) Annu. Rev. Immunol. 16, 293–332. (2) Darnell, J.E. (1997) Science 277, 1630–1635. (3) VanderKuur, J. et al. (1995) J. Biol. Chem. 270, 7587–7593. (4) Argetsinger, L. S. et al. (1995) J. Biol. Chem. 270, 14685–14692. (5) Zhu, T. et al. (1998) J. Biol. Chem. 273, 10682–10689. (6) Gauzzi, M. C. et al. (1996) J. Biol. Chem. 271, 20494–20500.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/12/J0900-20A_1.jpg