antibody

Insulin Regulated Aminopeptidase (IRAP, Placental Leucine Aminopeptidase, PLAP, Leucyl Cystinyl Aminopeptidase, LNPEP, Oxytocinase, Otase, Vesicle Protein of 165kD, vp165)

I8601-01B

100 ul

polyclonal

domestic rabbit

nonconjugated

Insulin Regulated Aminopeptidase (IRAP, Placental Leucine Aminopeptidase, PLAP, Leucyl Cystinyl Aminopeptidase, LNPEP, Oxytocinase, Otase, Vesicle Protein of 165kD, vp165)

rabbit

Pab

Antibodies

100 ul

IgG

IRAP (also known as LNPEP) was originally described as an insulin-responsive aminopeptidase found in GLUT4-containing vesicles, but later found to be a critical enzyme that regulates the expression and activity of several essential hormones and regulatory proteins, including the GLUT4 transporter. This membrane associated, zinc-dependent cystinyl aminopeptidase acts as both a receptor for angiotensin IV as well as the enzyme that catalyzes the synthesis of this essential hormone from its angiotensinogen precursor. IRAP catalyzes the hydrolysis of several peptide hormones, including oxytocin and vasopressin. Abnormal IRAP expression or activity is associated with several forms of cancer in humans, including renal and endometrial cancer. Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunofluorescence/Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000. Incubate membrane with diluted antibody in 1X TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:50. Immunofluorescence (IF-IC): 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Mo

Synthetic peptide corresponding to the sequence of human IRAP protein.

Recognizes human IRAP at ~165kD. Species Crossreactivity: mouse

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Not Determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not Determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to the sequence of human IRAP protein. Specificity: Recognizes human IRAP at ~165kD. Species Crossreactivity: mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

IF IP WB

-20°C

1. Garza, L.A. and Birnbaum, M.J. (2000) J Biol Chem 275, 2560-7, 2. Albiston, A.L. et al. (2001) J Biol Chem 276, 48623-6, 3. Keller, S.R. (2003) Front Biosci 8, s410-20, 4. Vanderheyden, P.M. (2009) Mol Cell Endocrinol 302,159-66, 5. Larrinaga, G. et al. (2007) Regul Pept 144, 56-61, 6. Suzuki, Y. et al. (2003) Clin Cancer Res 9, 1528-34. 7. Gross, D.N. et al. (2001) J Biol Chem 276, 48623-48626.

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