Interleukin 23R (Interleukin 23 Receptor, IL-23R) (PE) | US Biological I8443-49A product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Interleukin 23R (Interleukin 23 Receptor, IL-23R) (PE)

catalog :

I8443-49A

quantity :

100Tests

clonality :

monoclonal

host :

mouse

conjugate :

clone name :

7H8

reactivity :

human

product information

Catalog Number :

I8443-49A

Product wo Prefix :

Interleukin 23R (Interleukin 23 Receptor, IL-23R) (PE)

Host :

mouse

Product Type :

Mab

Conjugate :

Category :

Antibodies

Size1 :

100Tests

Clone # USB :

7H8

Isotype :

IgG2b

Desc1 :

IL-23R is an IL-23-specific binding protein that associates with IL-12 Rb1 to form a functional heterodimeric receptor complex. This complex mediates biological activities that are similar to, but distinct from, those induced by IL-12. IL-23R is expressed by Th1 and NK cells. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: Neat. 10ul labels 1x10e6 cells. Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Cells should be Fc-blocked by treatment with 1ug of human IgG/10e5 cells for 15 minutes at RT prior to staining. Do not wash excess blocking IgG from this reaction. 2. Transfer 25ul of the Fc-blocked cells (1x10e6 cells) or 50ul of packed whole blood to a 5ml tube. 3. Add 10ul of I8443-49A. 4. Incubate for 30-45 minutes at 4°C. 5. Following this incubation, remove unreacted I8443-49A reagent by washing the cells twice in 4ml of the same PBS buffer (Note: Whole blood will require an RBC lysis step at this point using any commercially available lysing reagent). 6. Finally, resuspend the cells in 200-400ul of PBS buffer for final flow cytometric analysis. 7. As a control for analysis, cells in a separate tube should be treated with PE-labeled mouse IgG2b antibody. Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C. Stable for 12 months after receipt at 4°C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.

Calc Crossreactivity :

Immunogen :

Recombinant protein corresponding to Gly24-Ile354 of human Interleukin 23 receptor extracellular domain, expressed in NS0 cells. UniProt Accession: Q5VWK5.

Specificity :

Recognizes human Interleukin 23R. Does not crossreact with recombinant mouse IL-23R.

Purity :

Purified by Protein A affinity chromatography from supernatant.

Form :

Supplied as a liquid in saline, ≤0.5% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).

Concentration :

~0.025mg/ml

Desc2 :

Product Type: Mab Isotype: IgG2b Clone No: 7H8 Host: mouse Source: human Concentration: ~0.025mg/ml Form: Supplied as a liquid in saline, ≤0.5% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE). Purity: Purified by Protein A affinity chromatography from supernatant. Immunogen: Recombinant protein corresponding to Gly24-Ile354 of human Interleukin 23 receptor extracellular domain, expressed in NS0 cells. UniProt Accession: Q5VWK5. Specificity: Recognizes human Interleukin 23R. Does not crossreact with recombinant mouse IL-23R. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

Storage Temperature :

4°C Do Not Freeze

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/29/I8443-49A_1.jpg