antibody

Interleukin 17E (IL-17E, Cytotoxic T-lymphocyte-associated antigen 8) (APC)

I8439-22E

100Tests

monoclonal

mouse

APC

7H5

Interleukin 17E (IL-17E, Cytotoxic T-lymphocyte-associated antigen 8) (APC)

mouse

Mab

APC

Antibodies

100Tests

7H5

IgG1

The Interleukin 17 (IL-17) family proteins, comprising six members (IL-17, IL-17B-IL through17F), are secreted, structurally related proteins that share a conserved cystine-knot fold near the C-terminus, but have considerable sequence divergence at the N-terminus. With the exception of IL-17B, which exists as a non-covalently linked dimer, all IL-17 family members are disulfide-linked dimers. IL-17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions. Human IL-17E cDNA encodes a 177aa precursor protein with a putative 32aa signal peptide. A second isoform of human IL-17E encoding a 161aa precursor protein also exists. The two isoforms differ in their signal peptide sequences. Mature human IL-17E shares 76% amino sequence identity with mature mouse IL-17E. Human IL-17E also shares from 25-36% aa sequence identity with the other human IL-17 family members. IL-17E expression was detected at very low levels by PCR in various peripheral tissues including brain, kidney, lung, prostate, testis, adrenal gland spinal cord and trachea. IL-17E binds and activates IL-17 B Receptor (IL-17B R) (alternatively known as IL-17 Rh1, IL-17E R, and EVI27), which is expressed in kidney and liver, and at lower levels in brain, testis and other endocrine tissues. The expression of IL-17B R is upregulated under inflammatory conditions. Ligation of IL-17E to IL-17 RB induces activation of nuclear factor kappa-B and stimulates the production of the proinflamatory cytokine IL-8. IL-17 has also been found to promote the expression of the prototypical Th2 genes. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul labels 10e6 cells. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Hu

Recombinant protein corresponding to Tyr33-Gly177 from human Interleukin 17E, expressed in E. coli (Q9H293).

Recognizes human Interleukin 17E. Cross-reacts with recombinant mouse IL-17E. Does not react with recombinant human IL-17, IL-17B, IL-17C, or IL-17F.

Purified by Protein G affinity chromatography from hybridoma culture supernatant.

Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).

~0.01mg/ml

Product Type: Mab Isotype: IgG1 Clone No: 7H5 Host: mouse Source: human Concentration: ~0.01mg/ml Form: Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC). Purity: Purified by Protein G affinity chromatography from hybridoma culture supernatant. Immunogen: Recombinant protein corresponding to Tyr33-Gly177 from human Interleukin 17E, expressed in E. coli (Q9H293). Specificity: Recognizes human Interleukin 17E. Cross-reacts with recombinant mouse IL-17E. Does not react with recombinant human IL-17, IL-17B, IL-17C, or IL-17F. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

FC

4°C Do not freeze

https://usbio-images.r.worldssl.net/prodimages/21/I8439-22E_1.jpg